Description
Key facts
Target:pCambia0380See target data,
Form:LyophilizedSee storage information,
Storage buffer:Constituents: 71% Tris, 17% EDTA
Product details:
Vector contains kanamycin resistance gene for bacterial selection, no gene for plant selection and no reporter gene.
The pCambia0380 vector offers:
High copy number in
E.coli
for high DNA yields
pVS1 replicon for high stability in
Agrobacterium
Small size
Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest
Bacterial selection with kanamycin
This vector contains a range of restriction sites on either side of the pUC8 polylinker, making it suitable for advanced construction purposes with users inserting their own promoters, selection genes, reporter genes. The only functional signals between the T-DNA borders are the start and stop codons, the histidine tag, and the NOS-poly(A) signal.
Genbank Sequence data
Concentration of Antibiotics for selection.
In bacterial system:
Kanamycin resistance (
aphIII
nptIII
/3'5''-aminoglycoside phosphotransferase typeIII from
Enterococcus faecalis
pJH1) in bacteria: use 50 μg/mL in
E.coli
A.tumefaciens
In plant system: no selection marker.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
