Description
Key facts
Purity:>95% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:CHO cells,
Tags:His tag C-Terminus,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Measured by its ability to induce activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells.The ED50 for this effect is typically 10-100ng/ml in the presence of 2 ng/mL recombinant mouse Wnt3a.,
Accession:Q9Z132,
Animal free:No,
Carrier free:No,
Species:Mouse,
Storage buffer:pH: 7.4Constituents: 100% PBS
Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RSPO1 also known as R-spondin 1 is a protein that plays an important role in the activation of the Wnt/β-catenin signaling pathway. It weighs approximately 29 kDa. RSPO1 is a secreted protein and is expressed in a variety of tissues including the ovary pancreas and regions of the brain. By binding to the leucine-rich repeat-containing G-protein-coupled receptors (LGRs) especially LGR4 LGR5 and LGR6 RSPO1 enhances Wnt signaling activity leading to increased stability and accumulation of β-catenin in the cell.
Biological function summary
RSPO1 acts as a major stimulator of cell proliferation and differentiation. It does not function alone but rather works in association with the Wnt receptors for signal enhancement. RSPO1 positively influences the regenerative processes in tissues and organs contributing to cell fate determination during development. As part of this interaction RSPO1 can reverse the degradation of Wnt receptors therefore amplifying the signaling required for proper developmental processes and tissue maintenance.
Pathways
RSPO1 integrates deeply into the Wnt/β-catenin signaling pathway where its role is important for amplifying the signals important for cellular activities such as proliferation and migration. RSPO1 interacts with pathway members such as Frizzled receptors and lipoprotein receptor-related proteins (Lrp5/6) to potentiate Wnt signaling. The function of RSPO1 in the Wnt pathway aligns with its interplay in epithelial cell stimulation and development making it an essential component in pathways driving cellular growth and differentiation.
RSPO1 has significant connections with congenital and proliferative conditions. Disruptions in RSPO1 expression are linked to sex reversal syndrome where the genetic sex does not match the phenotypic sex due to abnormal gonadal development. Additionally RSPO1 is implicated in colorectal cancer where upregulated Wnt signaling facilitated by RSPO1 promotes unchecked cellular proliferation. The interplay between RSPO1 and other pathway components like β-catenin and Frizzled receptors exacerbates these molecular dysfunctions highlighting its relevance in disease mechanisms.
