Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Cow,
Assay type:Sandwich (quantitative),
Sensitivity:= 420 pg/mL,
Range:420 - 7000 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Bovine IL-10 ELISA Kit (ab277386) is an
in-vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Bovine IL-10 serum (bovine IL-10 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Bovine IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and Bovine IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Bovine IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Bovine IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
