Description
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 21 pg/mL,
Range:20.5 - 5000 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human AMN ELISA Kit (ab277417) is an
in-vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human AMN in serum, plasma (collect plasma using EDTA and Citrate as an anticoagulant. Heparin is not recommended), and cell culture supernatants.
This assay employs an antibody specific for Human AMN coated on a 96-well plate. Standards and samples are pipetted into the wells and Human AMN present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human AMN antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human AMN bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
