Description
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 2 ng/mL,
Range:2.048 - 500 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human Cathepsin C ELISA Kit (ab277434) is an
in-vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human Cathepsin C in serum, plasma (collect plasma using citrate and heparin as an anticoagulant. EDTA is not recommended as anticoagulants for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for Human Cathepsin C coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Cathepsin C present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Cathepsin C antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Cathepsin C bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cathepsin C also known as dipeptidyl peptidase I is a lysosomal cysteine protease with a molecular mass of approximately 51 kDa. It functions mechanically by cleaving off dipeptides from the N-terminal end of proteins and peptides. Cathepsin C is widely expressed in various tissues with higher levels in immune cells like neutrophils and macrophages. This enzyme activates serine proteases from their inactive zymogen forms which is important for immune responses.
Biological function summary
The enzyme contributes significantly to the immune system. It forms part of a multi-enzyme complex that processes proenzymes such as granzymes and cathepsin G into their active forms. Through these actions Cathepsin C regulates cellular activities linked to inflammation and apoptosis. The proteolytic activity it provides is critical for the functioning of immune-related cells facilitating defense mechanisms against pathogens.
Pathways
Cathepsin C plays roles in the inflammatory and immune response pathways. It connects to the granzyme and lysosomal pathways important in processing and regulating granzyme activities critical for cytotoxic T lymphocyte functions. Cathepsin C maintains connections with proteins like granzymes and cathepsin G each involved in mediating immune responses and cellular death.
Cathepsin C is associated with conditions such as Papillon-Lefèvre Syndrome and aggressive periodontitis. These disorders arise due to disruptions in immune protection resulting from mutations in the Cathepsin C gene. The enzyme's abnormal activity in these diseases highlights its connection with proteins such as neutrophil elastase which play critical roles in tissue degradation processes leading to periodontal disease.
