Description
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The protease specific activity of ab277614 was 41701 pmol/min/mg at an enzyme concentration of 1.2 μM in a protease assay.,
Accession:P0DTD1,
Animal free:Yes,
Carrier free:No,
Species:SARS-CoV-2,
Storage buffer:pH: 7.5Preservative: 1.36% ImidazoleConstituents: 86.145% Water, 10% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 0.71% Sodium phosphate, 0.0015% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SARS 3CL protease also known as 3CL pro main protease (Mpro) or 3CL protein is a cysteine protease that plays an important role in the life cycle of coronaviruses including SARS-CoV and SARS-CoV-2. It has an approximate mass of 33.8 kDa. This enzyme is expressed in the cytoplasm of infected host cells. The protease cleaves the large polyprotein translated from viral RNA into functional proteins essential for viral replication and assembly.
Biological function summary
3CL pro functions as an important enzyme in the coronavirus replication process. It catalyzes the proteolytic cleavage of the viral polyprotein at 11 conserved sites which is essential for the release and maturation of non-structural proteins. These proteins are involved in forming the replicase-transcriptase complex necessary for viral RNA synthesis. 3CL pro does not function as part of a larger protein complex but interacts with other viral proteins during the replication process.
Pathways
3CL pro is integral to the viral replication and transcription pathway of coronaviruses. The activity of 3CL pro facilitates the processing of polyproteins which is a prerequisite for the assembly of the replication-transcription complex. This pathway includes interactions with RNA polymerase and helicase two proteins essential for RNA synthesis and unwinding respectively. The correct processing of viral proteins by 3CL pro ensures the production of functional complexes required for the life cycle of the virus.
SARS 3CL protease is linked to COVID-19 pathology caused by SARS-CoV-2 and is also of interest in the context of SARS. The protease is a target for antiviral drug development because it is necessary for viral replication. Inhibitors designed to disrupt the activity of 3CL pro could potentially halt the replication of SARS-CoV-2 within host cells. The relationships between 3CL pro and adjacent viral proteins such as RNA-dependent RNA polymerase are essential for understanding drug development strategies against coronaviral diseases.
