Description
Key facts
Cell type:THP-1,
Species or organism:Human,
Tissue:Blood,
Form:LiquidSee storage information,
Knockout validation:Western blot,
Disease:Acute Monocytic Leukemia
Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-ATG5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
APG5L commonly known as ATG5 or ATG5 protein plays a significant role in autophagy. This molecular component has a molecular weight of approximately 32 kDa. ATG5 is expressed in multiple tissue types with a higher expression profile in immune-related cells. The protein is essential for the formation of the autophagosome as it conjugates with ATG12 to form a complex that is critical for autophagosome membrane elongation.
Biological function summary
ATG5 plays an important part in the cellular process of autophagy affecting the degradation and recycling of cellular components. It is a part of a complex with ATG12 and ATG16L1 which facilitates the membrane building of the autophagosome. This protein is important for maintaining cellular homeostasis allowing the removal of damaged proteins and organelles and is involved in responses to stress and starvation.
Pathways
ATG5 is an important component in the autophagy pathway and is involved in cellular maintenance processes. This pathway is vital for the regulation of cell growth and metabolism. ATG5 works closely with other autophagy-related proteins such as LC3. The ATG5-ATG12 complex helps in the lipidation of LC3 which is critical for autophagosome maturation connecting its function to the mTOR signaling pathway that regulates many cellular processes.
Disruption in ATG5 function is linked to neurodegenerative diseases and cancer. For instance dysregulation of autophagy due to ATG5 dysfunction may result in the progression of neurodegenerative disorders like Alzheimer's disease. Additionally in certain cancers altered autophagy can influence the survival of cancer cells. The interplay between ATG5 and other proteins like p53 and Bcl-2 is under investigation to understand its role in these diseases.
