Description
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,
Disease:Carcinoma
Product details:
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
Recommended control
: Human wild-type A549 cell line (ab288558). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-AXIN1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Axin 1 alternatively known simply as axin is an important protein involved in cell signaling processes with a molecular weight of approximately 94 kDa. Axin 1 serves as a scaffold protein that plays a critical role in various signaling pathways. It shows expression in multiple tissues including the brain liver and kidney which indicates its involvement in various physiological processes. Due to its structural and functional properties axin 1 can be visualized and studied effectively even through techniques such as western blot.
Biological function summary
Axin 1 functions as a negative regulator within the Wnt signaling pathway by forming a destruction complex with other proteins. This complex is important in the degradation of beta-catenin thereby preventing excessive activation of Wnt target genes. It interacts with several components such as adenomatous polyposis coli (APC) and glycogen synthase kinase 3 beta (GSK3β) to facilitate these functions. The presence of axin 1 maintains the balance of signaling dynamics ensuring that cell proliferation and differentiation proceed correctly.
Pathways
Axin 1 plays a significant role in the control of the Wnt and JNK signaling pathways. Within the Wnt pathway it partners with proteins such as APC and GSK3β to regulate beta-catenin levels ensuring proper signal transduction. In the JNK pathway axin 1's interactions influence cell response to stress through modulation of signaling cascades. Its involvement in these pathways illustrates how axin 1 acts as a central component in cellular communication and response strategies.
Axin 1 associates with several conditions including colorectal cancer and hepatocellular carcinoma. Dysregulation of axin 1's function or expression can lead to aberrant Wnt signaling which is a pivotal factor in tumorigenesis linked with these cancers. Moreover proteins like beta-catenin and APC which interact with axin 1 also play a role in the progression of these diseases. Understanding axin 1's role provides insights into potential therapeutic strategies that target these pathways for effective treatment approaches.
