Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,Western blot,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Gene name-ERCC4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
XPF also known as ERCC4 is a protein that plays a mechanical role in DNA repair by cleaving DNA at junctions between single-stranded and double-stranded DNA. It exhibits a molecular mass of approximately 103 kDa. This protein is expressed in various tissues with high levels found in the liver and kidney. XPF functions as a DNA endonuclease significantly influencing genomic stability through its activity in DNA repair processes.
Biological function summary
The XPF protein collaborates with ERCC1 to form a heterodimeric complex important for nucleotide excision repair (NER). This complex is essential in recognizing and repairing bulky DNA adducts therefore maintaining DNA integrity. The complex performs specific incisions near DNA damage sites removing lesions that frequently occur due to environmental factors like UV radiation and chemical pollutants.
Pathways
XPF functions within the NER and interstrand crosslink repair pathways. In these pathways the XPF-ERCC1 complex coordinates with other proteins like XPA and RPA to accurately excise damaged DNA segments and facilitate repair synthesis. These interactions are important for restoring normal DNA configuration and function after damage thereby preventing mutations and genomic instability.
Mutations in XPF are linked to xeroderma pigmentosum group F (XP-F) and the Cockayne syndrome. XP-F is a disorder characterized by extreme sensitivity to sunlight and an increased risk of skin cancer due to impaired DNA repair capability. In Cockayne syndrome patients experience growth defects and neurological degeneration. Both of these disorders involve dysfunctional DNA repair mechanisms where proteins like ERCC1 and others in the repair pathways fail to adequately compensate for the defective XPF function.
