Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Tissue Homogenate, Serum,
Assay type:Quantitative,
Range:0.5 - 2.5 nmol/well
Product details:
This Free Fatty Acid Colorimetric Assay Kit (ab282927) provides a simple method for the quantification free fatty acids. The kit offers a convenient, sensitive, enzyme-based method for the detection of long-chain (C-8 (octanoate) and longer) fatty acids in various biological samples. In the EZScreenTM assay, fatty acids are converted to their CoA derivatives, which are subsequently oxidized by the enzyme mix to yield an intermediate. Formed intermediate then reacts with the Free Fatty acid probe to generate color which can be read by a spectrophotometer at OD 570 nm. Our 384-well format enables analyses of large number of samples on a single plate in a high throughput mode. Sample volumes between 1 and 10 μl can be used with this kit.
Sample types:
Serum, plasma, and other body fluids. Animal tissues, Cell culture: adherent or suspension cells, Growth media and foods.
This product is manufactured by BioVision, an Abcam company and was previously called K956 EZScreen™ Free Fatty Acid Colorimetric Assay Kit (384-well). K956-400 is the same size as the 400 test size of ab282927.
Fatty Acids play important roles in normal metabolism and in many disease states. They are precursors to a number of bioactive classes of compounds including prostaglandins and leukotrienes, and have been implicated to play roles in such diverse functions as the immune system, autism, and the inflammatory response. Fatty acid levels are an important measure of the physiologic state. High levels are associated with diabetes mellitus, obesity, endocrine dysfunctions, and metabolic syndrome.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Free fatty acids (FFAs) also known as non-esterified fatty acids (NEFAs) play important roles in various cellular functions. These molecules found in both serum and plasma are primarily unbound to glycerol and circulate in the bloodstream. Through their small molecular size typically low molecular mass free fatty acids contribute to metabolic processes and energy homeostasis. They are expressed in various tissues throughout the body especially in adipose tissues and are released during the breakdown of triglycerides by lipases.
Biological function summary
Free fatty acids function as key energy substrates and signaling molecules in cellular metabolism. They are part of complex metabolic processes that influence insulin signaling inflammation and lipid metabolism. FFAs also serve as important substrates for β-oxidation which occurs in the mitochondria providing energy in the form of ATP. Furthermore they act as ligands to nuclear receptors such as PPARs (peroxisome proliferator-activated receptors) which regulate genes involved in lipid metabolism.
Pathways
Free fatty acids are central to lipid metabolism and insulin signaling pathways. They play a significant role in the regulation of the AMPK (AMP-activated protein kinase) pathway affecting energy balance and metabolism. In the lipid metabolism pathway they are associated with enzymes like acetyl-CoA carboxylase and carnitine palmitoyltransferase which are important for fatty acid synthesis and oxidation. The interaction of free fatty acids with these pathways illustrates their regulatory functions in maintaining energy homeostasis.
Free fatty acids are linked to metabolic syndromes and cardiovascular diseases. Elevated levels of FFAs in the bloodstream can lead to insulin resistance an event involved in the pathogenesis of type 2 diabetes. High FFA levels can further contribute to lipotoxicity which is associated with heart diseases. The protein adiponectin is commonly associated with FFA-related disorders; it modulates FFA metabolism and is often reduced in individuals suffering from these metabolic disorders.
