Description
Key facts
Detection method:Colorimetric,
Sample types:Tissue, Suspension cells, Adherent cells,
Assay type:Quantitative,
Sensitivity:= 0.025 µg
Product details:
This Glycogen Colorimetric Assay Kit (ab282931) is an easy and convenient assay to measure glycogen levels in biological samples. In the assay, glucoamylase hydrolyzes the glycogen releasing glucose which is then specifically oxidized to produce a product that reacts with OxiRed Probe to generate color (OD 590 nm). The method is quantitative, rapid, simple, sensitive, and designed for high throughput format. The assay can detect as low as 0.025 μg of glycogen in 384-well assay format.
Sample Type
: Animal tissues such as liver etc and Cell culture: adherent or suspension cells
This product is manufactured by BioVision, an Abcam company and was previously called K960 EZScreen™ Glycogen Colorimetric Assay Kit (384-Well). K960-400 is the same size as the 400 test size of ab282931.
Glycogen is a branched polysaccharide, made of glucose units that serves as the primary source of energy storage in animals. Most of the glucose residues in glycogen are linked by α-1,4-glycosidic bonds with branching via α-1,6 linkage. In mammals, the two major sites of glycogen storage are liver and skeletal muscle. In liver, glycogen synthesis and degradation are regulated to maintain blood-glucose levels, while stored glycogen in muscle can be processed to meet the energy needs of the muscle itself. Abnormal ability to utilize glycogen is found in patients suffering diabetes and other genetic glycogen storage related diseases.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glycogen acts as a critical energy storage polymer in the human body. It is a branched glucose polymer commonly referred to as animal starch which forms granular aggregates within cells. These aggregates typically weigh between 400 to 600 kDa. Glycogen is primarily expressed in the liver and skeletal muscle tissues. In the liver glycogen maintains blood glucose levels while in muscles it supplies energy during contraction. Measurement of glycogen levels in tissues can be performed using specialized tools like a glycogen assay kit glycogen x or a glycogen test.
Biological function summary
Glycogen functions to store energy in the form of glucose units allowing for a quick release when the body requires immediate energy. It does not belong to a traditional complex but does interact with various enzymes in glycogen metabolism. Glycogen phosphorylase and glycogen synthase regulate its breakdown and synthesis respectively. Enzymatic activities such as the hydrolysis of glycogen play a significant role in mobilizing glucose units from glycogen therefore facilitating metabolic processes.
Pathways
Glycogen is integral in glycolysis and gluconeogenesis. During glycolysis enzymes such as phosphoglucomutase convert glycogen-derived glucose-1-phosphate into glucose-6-phosphate which then enters glycolysis to produce energy. In gluconeogenesis glycogen can be synthesized as an energy reserve. Glycogen metabolism involves key proteins like insulin and glucagon that control glucose levels and consequently the activities of enzymes acting on glycogen.
Glycogen storage diseases (GSD) and type 2 diabetes illustrate the consequences of abnormal glycogen metabolism. The accumulation or deficiency of glycogen in tissues causes various forms of GSDs which lead to organ dysfunction. In diabetes impaired insulin action affects glycogen synthesis and breakdown resulting in dysregulated blood sugar levels. Key proteins associated with these conditions include insulin glucagon and glycogen synthase.
