Description
Key facts
Detection method:Colorimetric,
Sample types:Inhibitor compounds,
Assay Platform:Microplate (12 x 8 well strips)
Product details:
Alpha-Amylase Inhibitor Screening Kit (Colorimetric) (ab283391) can be used to screen/characterize potential inhibitors of Alpha-Amylase.
Alpha-Amylase enzyme is responsible for the breakdown of complex carbohydrates in humans. Thus, inhibition of alpha-amylase could be considered as a strategy for the treatment of disorders related to carbohydrate uptake, such as diabetes, obesity, dental cavities and periodontal diseases. In this kit human Alpha-Amylase hydrolyzes the synthetic substrate, yielding smaller fragments containing the chromophore (pNP; OD = 405 nm). A potent, specific inhibitor is also included in the kit.
The 1-step assay is simple and can be completed within 1 hour.
Other Notes
This product was previously called K482 a-Amylase Inhibitor Screening Kit. Biovision was acquired by Abcam in 2021.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pancreatic alpha amylase also known as alpha-amylase P or AMY2A and salivary alpha amylase (AMY1) are enzymes that play essential roles in carbohydrate digestion. Mechanically they hydrolyze alpha-14-glycosidic linkages in starches breaking them down into smaller sugars like maltose and dextrins. Pancreatic alpha amylase has a molecular mass of approximately 56 kDa. The pancreas and salivary glands predominantly express these enzymes facilitating digestive processes in the small intestine and oral cavity respectively.
Biological function summary
These alpha-amylases initiate the digestive process for dietary starches and glycogen by breaking them into sugars which are then more easily absorbed by the body. While they function largely independently their activity is supported by other digestive enzymes that further process carbohydrates throughout the digestive system. This enzymatic action is important for maintaining energy supply from glycogen and starches highlighting the significant role of alpha amylase in metabolic nutrition.
Pathways
The catalytic action of pancreatic and salivary alpha amylase is a part of the digestive pathway for carbohydrates where it accelerates the breakdown of starches. These enzymes interact within the amylase pathway which includes other related enzymes like alpha-glucosidases that sequentially digest disaccharides into glucose. They are not known to form permanent complexes but work in conjunction with these digestive enzymes to facilitate efficient energy release.
Alterations in alpha-amylase activity have connections to conditions like pancreatitis and salivary gland dysfunctions where changes in enzyme levels provide important diagnostic information. Conditions such as pancreatitis an inflammation of the pancreas link directly to abnormal amylase activity and concentration. Additionally they associate with other proteins involved in inflammation and stress responses which are measured in clinical settings to assess pancreatic and salivary gland health.
