Description
Key facts
Detection method:Fluorescent,
Sample types:Inhibitor compounds,
Assay Platform:Microplate (12 x 8 well strips)
Product details:
COX2 Inhibitor Screening Kit (Fluorometric) (ab283401) offers a rapid, simple, sensitive, and reliable test for measuring COX-2 inhibitors.
In the COX2 assay, the enzyme COX-2 generates Prostaglandin G2 (PGG2), an intermediate product in the COX pathway; the intensity of this signal decreases when a COX-2 inhibitor is present, allowing for the identification of potential COX-2 inhibiting compounds by comparing the fluorescence levels in the presence and absence of a test compound.
COX2 assay protocol summary:
- Add samples and standards to wells
- Add reaction mix
- Analyze with fluorescence microplate reader (Ex/Em = 535/587 nm)
Other Notes
This product was previously called K547 Biovision COX-2 Inhibitor Screening Kit (Fluorometric). Biovision was acquired by Abcam in 2021.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cyclooxygenase-2 (COX-2) also known as prostaglandin-endoperoxide synthase 2 is an enzyme with a mass of about 72 kDa. It is a vital component in the conversion of arachidonic acid to prostaglandins which are lipid compounds that exhibit a variety of physiological functions. COX-2 is an inducible enzyme meaning its expression can be increased by various factors such as cytokines and growth factors. It is typically present in inflammatory sites as well as in the brain kidney and other tissues under specific conditions.
Biological function summary
COX-2 participates in processes related to inflammation and pain. Although it primarily functions as an individual enzyme it can interact with other proteins in tissue-specific contexts. COX-2 is particularly important in situations requiring an increased prostaglandin production like inflammatory responses and the healing of wounds. It contributes to the regulation of normal physiological functions including renal hemodynamics and the regulation of blood flow within the kidney.
Pathways
The COX-2 enzyme plays a substantial role in the arachidonic acid metabolic pathway. It engages in this pathway by facilitating the biosynthesis of prostaglandins from arachidonic acid. COX-2 operates alongside other proteins such as COX-1 which is its constitutively expressed counterpart as well as other enzymes involved in prostaglandin synthesis like lipoxygenases. This shared pathway highlights the coordinated efforts required in regulating inflammation and maintaining tissue homeostasis.
COX-2 has been closely linked to conditions such as arthritis and cancer. In arthritis COX-2 contributes to the chronic inflammation and pain observed in affected joints. In cancer overexpression of COX-2 has been observed in various tumor types where it may promote tumor growth and inhibit apoptosis. Celecoxib a COX-2 inhibitor is often used to reduce disease symptoms by targeting COX-2's role in these pathological processes. Through its involvement in these disorders COX-2 also interacts indirectly with other critical proteins like nuclear factor kappa B (NF-kB) that participate in inflammatory and tumor pathways.
