Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Tissue Extracts, Serum, Cell Lysate,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.45 ng/mL,
Range:1.25 - 80 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
The Huamn Calreticulin-3 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is designed for detection of Calreticulin 3 (CALR3) in human plasma, serum, cell culture, and cell lysate samples.
This assay employs a quantitative sandwich enzyme immunoassay technique that measures human CALR3 in approximately 4 hours. A polyclonal antibody specific for human CALR3 has been pre-coated onto a 96-well microplate with removable strips. CALR3 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human CALR3, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
