Description
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Serum,
Assay type:Sandwich (quantitative),
Sensitivity:= 4.5 pg/mL,
Range:6.14 - 1500 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
The COVID-19 S-Protein (S1RBD) ELISA Kit (ab284402) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of COVID-19 S-Protein (S1RBD) in serum (plasma is not recommended in this assay) and cell culture supernatants. This assay employs an antibody specific for COVID-19 S-Protein (S1RBD) coated on a 96-well plate. Standards and samples are pipetted into the wells and COVID-19 S-Protein (S1RBD) present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-COVID-19 S-Protein (S1RBD) antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of COVID-19 S-Protein (S1RBD) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The COVID-19 Spike Protein often referred to as SARS-CoV-2 Spike Protein is an important structural protein of the virus with a molecular mass around 180 kDa. This protein facilitates viral entry into host cells by binding to the angiotensin-converting enzyme 2 (ACE2) receptor which is primarily expressed in the respiratory tract. It's a trimeric protein composed of S1 and S2 subunits. The S1 subunit contains the receptor binding domain while the S2 subunit mediates membrane fusion.
Biological function summary
The spike protein plays an important role in viral infectivity and host range determination. The spike protein is part of the larger viral particle complex that includes other structural proteins like the nucleocapsid protein. Its interaction with host cell receptors triggers conformational changes allowing viral entry and subsequent replication within the host cells. This process is pivotal for the virus's lifecycle.
Pathways
The spike protein is integral to the viral entry pathway a critical step in the viral lifecycle. It intersects with the renin-angiotensin system (RAS) pathway through its interaction with the ACE2 receptor. This interaction not only facilitates viral entry but can also dysregulate the RAS pathway. Additionally the spike protein's activity is related to other viral proteins that assist in replication and assembly such as the envelope protein.
The spike protein is directly linked to COVID-19 the disease caused by SARS-CoV-2 infection. Its interaction with the ACE2 receptor has been associated with severe respiratory illnesses and potentially other conditions like cardiovascular disorders due to its effect on the RAS pathway. The nucleocapsid protein is also important in the virus's pathogenesis working together with the spike protein to establish infection and disease progression.
