Description
Key facts
Detection method:Fluorescent,
Sample types:Inhibitor compounds,
Assay type:Enzyme activity (quantitative),
Assay Platform:Microplate (12 x 8 well strips)
Product details:
This Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (ab284511) (K797) offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of MAO-B inhibitors.
The assay is based on the fluorometric detection of H
, one of the byproducts generated during the oxidative deamination of MAO substrate (Tyramine).
This product is manufactured by BioVision, an Abcam company and was previously called K797 Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric). K797-100 is the same size as the 100 test size of ab284511.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Monoamine Oxidase B (MAO-B) also known as MAOB or MAO-B inhibitor is an enzyme that is a part of the flavin-containing amine oxidases group. It exists as a homodimer with each monomer having a mass of about 58 kDa. This enzyme plays an important role in the catabolism of monoamines by oxidizing neurotransmitters such as dopamine in the brain. MAO-B is expressed mainly in the outer membrane of mitochondria in neuronal and glial cells and to a lesser extent in other tissues like the liver.
Biological function summary
The enzyme contributes to the breakdown of biogenic and dietary amines supporting neurotransmitter regulation and signal modulation. It does not work as part of a larger protein complex but has important interactions within its local cellular environment. MAO-B helps maintain the proper levels of monoamine neurotransmitters preventing their accumulation and potential neurotoxicity.
Pathways
Monoamine Oxidase B functions in the dopamine and serotonin metabolic pathways where it actively participates in the oxidative deamination of monoamines. In these pathways MAO-B acts alongside other proteins such as Monoamine Oxidase A (MAO-A) which shares similar functions and substrate preferences. This enzyme activity helps modulate mood and emotional behaviors which are critical for normal brain function.
Monoamine Oxidase B has a significant connection with neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. In Parkinson's disease MAO-B intensifies the oxidative stress by promoting dopamine breakdown which is linked to neuronal degeneration. In Alzheimer's disease elevated MAO-B activity may contribute to the overproduction of neurotoxic metabolites. Through these conditions MAO-B is often targeted by MAO-B inhibitors to reduce its activity alleviating symptoms and slowing disease progression.
