Description
Key facts
Detection method:Colorimetric,
Sample types:Inhibitor compounds,
Assay type:Enzyme activity (quantitative),
Assay Platform:Microplate (12 x 8 well strips)
Product details:
The alpha-Glucosidase Inhibitor Screening Kit (Colorimetric) ab284520 is a convenient assay for the screening/characterizing Alpha-Glucosidase inhibitors.
How the assay works
The alpha-Glucosidase assay uses the ability of an active Alpha-Glucosidase to cleave a synthetic substrate thus, releasing a chromophore (OD: 410 nm). In the presence of an Alpha-Glucosidase specific inhibitor, the enzymatic activity is greatly reduced which is detected by a decrease of absorbance readings.
Alpha-Glucosidase assay summary
- Add samples and standards to wells
- Add reaction mix and incubate
- Analyze with microplate reader at OD: 410 nm in kinetic mode for 60 min at room temperature.
Other Notes
This product was previously called K938 Biovision a-Glucosidase Inhibitor Screening Kit (Colorimetric). Biovision was acquired by Abcam in 2021.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
