Description
Key facts
Sample types:Plasma, Tissue Homogenate, Serum, Other biological fluids,
Assay type:Competitive,
Sensitivity:< 0.94 ng/mL,
Range:1.563 - 100 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
8-hydroxy-2'-deoxyguanosine (8-OHdG) ELISA Kit (ab285254, K4160) is used for quantitative measurement of 8-OHdG in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
This product is manufactured by BioVision, an Abcam company and was previously called K4160 8-hydroxy-2'-deoxyguanosine (8-OHdG) ELISA Kit. K4160-100 is the same size as the 1 x 96 tests size of ab285254.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
8-Hydroxy-2'-deoxyguanosine (8-OHdG) also known as 8-hydroxyguanine or simply 8-OHdG is a well-known marker of oxidative DNA damage. This compound forms through the oxidative modification of deoxyguanosine a nucleoside component of DNA mainly by reactive oxygen species (ROS). 8-OHdG is a stable product with a molecular mass of 283.26 g/mol. It is found in various tissues and is excreted in urine which makes it a useful non-invasive biomarker for assessing oxidative stress levels in the body.
Biological function summary
8-hydroxy-2-deoxyguanosine plays a significant role in indicating cellular oxidative damage but it doesn't act as a signaling molecule or enzyme. Instead it serves as a biomarker that results from the damage. Oxidative stress caused by reactive oxygen species can lead to the formation of 8-OHdG and indicates potential mutations that may arise if the damage goes unrepaired. Its formation does not involve complex protein assemblies but results from the attack on DNA by ROS highlighting cellular environments exposed to oxidative conditions.
Pathways
Several factors cause oxidative stress leading to the generation of 8-OHdG linking it to key biological pathways such as the DNA damage response and repair pathways. The base excision repair (BER) pathway is important for recognizing and repairing the damage introduced by 8-OHdG. Proteins such as 8-oxoguanine glycosylase (OGG1) directly interact with 8-OHdG during the repair process excising the damaged base from DNA to prevent mutations and maintain genomic integrity.
Researchers associate elevated levels of 8-OHdG with various conditions linked to oxidative stress such as cancer and neurodegenerative diseases like Alzheimer's disease. In cancer the accumulation of 8-OHdG can provide evidence of increased oxidative DNA damage which may lead to mutations and carcinogenesis. Alzheimer’s disease patients often exhibit elevated 8-OHdG levels indicating oxidative damage in neural tissues. The protein OGG1 plays a pivotal role in relationship with these diseases serving as an important enzyme for repairing the 8-OHdG lesions and potentially impacting disease progression.
