Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Tissue Homogenate, Serum, Other biological fluids,
Reacts with:Human,
Assay type:Competitive,
Sensitivity:< 18.75 pg/mL,
Range:31.25 - 2000 pg/mL,
Assay Platform:Microplate (12 x 8 well strips)
Product details:
Human Angiotensin II ELISA Kit ab285306 is a competitive ELISA to measure Human Angiotensin II in serum, plasma, tissue homogenate, other biological fluids with a sensitivity of 18.75 pg/mL.
How the assay works:
The microtiter plate provided in this kit has been pre-coated with the target antigen. During the reaction, the target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. The unbound sample or excess conjugate are washed from the plate. HRP-Streptavidin (SABC) is added and incubated. Then TMB substrate solution is added to each well. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is measure at 450 nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Angiotensin II also known as Ang II is a potent peptide hormone involved in various physiological activities. It has a mass of approximately 1 kDa. Angiotensin II is formed from angiotensin I through the action of the enzyme angiotensin-converting enzyme (ACE). This peptide primarily expresses in the blood vessels adrenal gland and kidney. It binds mainly to angiotensin II receptor type 1 (AT1 receptor) and type 2 (AT2 receptor) to mediate its effects.
Biological function summary
This peptide hormone regulates blood pressure and fluid balance. It acts as part of the renin-angiotensin system (RAS) and triggers vasoconstriction which increases blood pressure. By stimulating the release of aldosterone it facilitates sodium and water retention further affecting blood volume. The regulation of these mechanisms does not occur in isolation; Angiotensin II integrates with other signaling molecules like reactive oxygen species to exert its functions.
Pathways
The renin-angiotensin system is the primary pathway where Angiotensin II plays an important role. It connects closely with the endothelial and inflammatory pathways influencing cardiovascular health. It interacts with various proteins in these pathways including ACE and the AT1 and AT2 receptors which are critical for its effects on vascular tone and cellular proliferation. Additionally through the RAS Angiotensin II cross-talks with the kinin-kallikrein system which balances vasoconstrictive and vasodilatory actions.
Angiotensin II is linked to hypertension and heart failure. Elevated levels can contribute to increased blood pressure promoting vascular and cardiac remodeling through interactions with aldosterone and endothelin-1. In heart failure Angiotensin II exacerbates the progression of the disease by promoting fibrosis and hypertrophy via the AT1 receptor. The modulation of Angiotensin II activity through ACE inhibitors or receptor blockers is therapeutic in treating these conditions highlighting the peptide's critical role in these pathological states.
