Description
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,Western blot,
Mutation description:Knockout,
Disease:Carcinoma
Product details:
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
Recommended control
: Human wild-type A549 cell line (ab288558). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-GATA3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GATA3 also known as GATA-binding factor 3 is a transcription factor with a molecular mass of about 50 kDa. It belongs to the GATA family of zinc finger transcription factors. GATA3 is mainly expressed in the immune system especially in T-cells as well as in the nervous system and mammary glands. It plays a role in the regulation of critical developmental processes and cell lineage commitment through its binding to the 'GATA' motif in the promoter regions of target genes.
Biological function summary
GATA3 functions as a regulator of gene expression involved in various differentiation processes. It is not a solitary molecule; it interacts with other proteins to form transcriptional complexes. In T-helper type 2 (Th2) cells GATA3 is necessary for Th2 cell differentiation and cytokine production. Its expression is vital for the development of the nervous system and mammary gland morphogenesis highlighting its role in diverse biological contexts.
Pathways
GATA3 plays a significant role in the Th2 differentiation pathway and is linked to the immune response. It acts in association with other transcription factors like STAT6 and T-bet. GATA3 promotes the transcription of Th2 cytokines such as IL-4 IL-5 and IL-13 while suppressing Th1 responses. This balance orchestrated by GATA3 is important for immune homeostasis and the body's ability to respond to infections and allergens.
The dysregulation of GATA3 is associated with atopic diseases and breast cancer. Aberrant expression of GATA3 is often observed in allergic conditions due to its role in Th2 cell differentiation and cytokine expression. In the context of breast cancer GATA3 levels can impact tumor progression and are correlated with estrogen receptor status. Additionally its relation with other breast cancer-related proteins like L50 underlines its importance in cancer biology and potential as a therapeutic target.
