Description
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 2.48 pg/mL,
Range:10.94 - 700 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human Cathepsin Z ELISA kit (ab287165) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Cathepsin Z protein in human serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cathepsin Z with 2.48 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cathepsin Z also known as cathepsin X or CTSZ is a lysosomal cysteine protease with a molecular weight of approximately 34 kDa. It is expressed mainly in immune cells such as macrophages and dendritic cells but also found in other tissues including liver kidney and spleen. This protease performs proteolytic functions which involve cleaving peptide bonds in proteins contributing to protein catabolism in cells. The widespread expression of Cathepsin Z suggests its involvement in various physiological processes.
Biological function summary
Cathepsin Z participates in immune response regulation and cellular adhesion processes. It belongs to the papain-like cysteine protease family. Notably it associates with integrins to influence cell-extracellular matrix interactions affecting cell migration and adhesion. By modulating these processes Cathepsin Z impacts immune surveillance and tissue remodeling demonstrating its multifaceted role in maintaining cellular homeostasis.
Pathways
Cathepsin Z engages significantly in apoptotic and antigen-presenting pathways. In apoptosis it influences cell death by degrading specific substrates involved in cell survival. It also assists in the processing of antigens within dendritic cells facilitating effective antigen presentation to T cells. Related proteins such as Cathepsin S and Cathepsin L which also partake in lysosomal pathways further illustrate the shared functionality and redundancy within this protease family enabling a robust regulatory network.
Cathepsin Z has implications in cancer progression and neurodegenerative diseases. It is often overexpressed in cancerous tissues where it aids tumor cell invasion and metastasis through degradation of extracellular components. Additionally alterations in Cathepsin Z activity are linked to Alzheimer's disease where it may affect the processing of amyloid precursor proteins similar to its interaction with other proteins like Cathepsin B. These connections highlight the potential for Cathepsin Z as a target for therapeutic intervention in various pathological conditions.
