Description
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,Sanger Sequencing,Western blot,
Disease:Carcinoma
Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-FANCA, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The FANCA protein also known as FAA protein is a critical component in the DNA repair mechanism. FANCA is approximately 162 kDa and shows widespread expression in the human body particularly in proliferating cells. This protein plays an essential role in maintaining genomic stability by participating in the repair of DNA interstrand crosslinks. FANCA's primary function involves its interaction with other proteins in the cell nucleus where DNA repair and replication processes occur.
Biological function summary
FANCA works as a part of the Fanconi anemia (FA) core complex involving around 13 proteins. This complex plays an important role in the FA DNA repair pathway facilitating the repair of interstrand DNA crosslinks. FANCA acts by orchestrating the recruitment and activation of downstream repair proteins which ensures the high-fidelity maintenance of the genetic material during cell division. Dysfunction in the FA complex where FANCA operates can lead to chromosomal instability and increased mutation rates.
Pathways
FANCA functions prominently in the Fanconi anemia pathway and is also involved in the homologous recombination repair pathway. In the FA pathway FANCA works closely with related proteins like FANCB and FANCC to initiate the DNA repair process. FANCA's interaction with these proteins allows it to execute its repair role effectively ensuring cellular genomic integrity. These pathways are critical for protecting cells from DNA damage during replication and shielding them from cancer development.
FANCA mutations are directly associated with Fanconi anemia a genetic disorder characterized by marrow failure cancer predisposition and various congenital abnormalities. Loss of FANCA function disrupts the repair pathway leading to cellular hypersensitivity to DNA-damaging agents. Additionally FANCA's impairment has been implicated in certain cancers like acute myeloid leukemia where its interaction with other FA proteins gets compromised leading to tumor progression.
