Description
Key facts
Cell type:HCT116,
Species or organism:Human,
Tissue:Colon,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,Western blot,
Disease:Carcinoma
Product details:
Recommended control
: Human wild-type HCT116 cell line (ab288559). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-HOXA9, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HOXA9 also known as Homeobox A9 functions as a transcription factor and plays an important role in regulating gene expression during embryonic development and hematopoiesis. The protein has a mass of approximately 27 kDa. It shows expression in hematopoietic progenitor cells as well as in certain tissues like the lungs and the central nervous system. By binding to specific DNA sequences HOXA9 regulates genes that are vital for cellular differentiation and proliferation.
Biological function summary
HOXA9 controls the transition and lineage commitment of hematopoietic stem cells. The protein often interacts as part of a transcriptional complex involving other HOX family proteins to maintain proper gene expression balance. This regulatory function is essential for normal gene expression patterns needed in stem cell differentiation and organ development processes.
Pathways
HOXA9 plays significant roles in the hematopoietic cell lineage and the retinoic acid signaling pathway. Through these pathways it collaborates with other HOX proteins like HOXB4 to maintain stem cell properties and influence the differentiation process. These interactions facilitate the production of necessary blood cell types and ensure effective response mechanisms to various developmental cues.
HOXA9 shows a strong connection with acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS). Overexpression or mutation of HOXA9 disturbs normal hematopoietic processes often leading to these malignancies. The interaction with protein PBX1 also highlights its role in these diseases as their joint involvement frequently appears in leukemogenesis. Understanding how HOXA9 contributes to these pathways is critical for developing targeted therapeutic strategies.
