Description
Key facts
Detection method:Flow cytometry-fluorescent,
Sample types:Suspension cells, Adherent cells,
Reacts with:Mammals,
Assay type:Cell-based,
Assay Platform:Flow cytometer, Fluorescence microscope
Product details:
The EdU DNA Synthesis Monitoring Kit ab287859 (previously known as EZClick EdU DNA Synthesis Monitoring Kit, K946) utilizes a novel approach that relies on incorporation of 5-EdU (5-ethynyl-2’deoxyuridine) as nucleoside analog to thymidine into newly synthesized DNA directly in the cell culture. Incorporation of EdU into genomic DNA in S-phase is detected based on a click reaction between the alkyne moiety of EdU and fluorescent azide. Compared to historically used BrdU, click reaction is carried in mild conditions and flow cytometry/fluorescence microscopy can be used for assessment of proliferating cells in the population. Our kit provides sufficient materials for 100 assays based on the protocol below.
Applications:
Detection of DNA synthesis in proliferating cells and assessment of cell cycle phase
Screening for genotoxic compounds and effectors of cell division cycle
Evaluating effects of anti-cancer drugs and genotoxic agents
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
