Description
Key facts
Detection method:Colorimetric,
Sample types:Food samples, Saliva, Tissue,
Assay type:Quantitative,
Assay Platform:Microplate
Product details:
Total Glycosaminoglycans Assay Kit (ab289842, K2085) can measure the total amount of Glycosaminoglycans (GAGs) including sulfated GAGs such as chondroitin sulfate, heparan sulfate etc. and non-sulfated GAGs such as hyaluronic acid in various biological samples. In this assay, GAGs interacts with a specific probe to form a colored product that is measured by absorbance at 400 nm. The colored signal is directly proportional to the GAG concentration in the sample. The kit offers a simple, rapid, sensitive and convenient way to measure the total amount of GAGs in various sample types. It can detect as low as 1 μg of chondroitin sulfate or hyaluronic acid under the assay conditions.
Glycosaminoglycans (GAGs) or mucopolysaccharides are long, linear, negatively chargedpolysaccharides consisting of repeating disaccharide units. Based on their core disaccharidestructure, GAGs are categorized into four groups, sulfated GAGs including heparin/heparansulfate, chondroitin sulfate, keratan sulfate and non-sulfated GAGs including hyaluronic acid,respectively. They are the most abundant heteropolysaccharides in the human eye. They are akey constituent of the extracellular matrix and act as a filler substance between cells and fibersin tissues. They vary in molecular mass (10-100 kDa), disaccharide construction, sulfation and havediverse functions.
GAGs are the structural scaffolds and provide support and adhesiveness inbone, corneas, skin and connective tissues. Additionally, GAGs play an important role in manybiological processes including cell signaling, cell growth and proliferation, anti-coagulation,angiogenesis, tumor progression, etc. GAGs are degraded in the lysosome via four differentpathways. However, patients with the lysosomal storage disorder known asmucopolysaccharidoses (MPSs) have impaired lysosomal break down of GAGs and hence havehigher levels of GAGs in their tissues or urine. Abnormal accumulation of GAGs in cells, blood andconnective tissues over time can lead to permanent cell damage that can affect appearance,movement and functioning of organs.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
