Description
Key facts
Detection method:Colorimetric,
Sample types:Serum, EDTA Plasma, Heparin Plasma, Citrate plasma, Cell culture media,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 18.846 pg/mL,
Range:58.594 - 3750 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human C7 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of C7 protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human C7 with 18.846 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Complement component 7 (C7) with a molecular mass of approximately 93 kDa plays an essential mechanical role in the complement system a critical part of innate immunity. C7 is one of the late components of the complement cascade involved in the formation of the membrane attack complex (MAC). It is primarily expressed in the liver and found in the blood plasma. By associating with other components C5b and C6 C7 anchors into the lipid bilayer of target cells serving as a foothold for further MAC assembly.
Biological function summary
C7 functions as an important facilitator in the innate immune response being an integral part of the membrane attack complex (MAC). This complex forms transmembrane channels in the cell membrane of pathogens leading to their lysis and destruction. Through its interaction with complement components like C5b and C6 C7 enables the insertion and stabilization of the MAC. This process is important for the immune system’s ability to eliminate pathogens quickly and effectively protecting the host from microbial invaders.
Pathways
The complement system pathway is the primary biological pathway involving C7. Within this pathway C7 interacts closely with complement proteins C5 and C6 contributing to the terminal pathway that leads to pathogen lysis. Besides it is linked with the classical and alternative complement pathways both of which converge at the activation of C5. Through these interactions and processes C7 works synergistically with other complement proteins to execute robust immune defense mechanisms.
C7 deficiencies have been associated with an increased susceptibility to infections particularly by Neisseria species due to impaired MAC formation. This link highlights the importance of C7 for adequate host defense. Additionally overactivation of the complement system potentially involving C7 contributes to the pathology of autoimmune disorders like systemic lupus erythematosus. In such conditions dysregulation involving proteins like C5b and C6 can lead to tissue damage and inflammation further illustrating the importance of C7 in maintaining immune homeostasis.
