Description
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Serum, Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.45 ng/mL,
Range:0.45 - 100 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human ADAMTS15 ELISA kit is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of human ADAMTS15 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human ADAMTS15 coated on a 96-well plate.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ADAMTS15 also known as A Disintegrin And Metalloproteinase with Thrombospondin motifs 15 is a member of the ADAMTS family of zinc-dependent metalloproteases. It is involved in the degradation of extracellular matrix components especially proteoglycans. This protease has a mass of approximately 110 kDa. Researchers found ADAMTS15 expression mainly in cartilage and testis highlighting its tissue-specific roles. Understanding its mechanical role as a proteoglycanase gives insight into its contribution to tissue remodeling dynamics.
Biological function summary
ADAMTS15 plays a significant role in extracellular matrix organization and turnover. It mediates proteolytic processing of aggrecan and other proteoglycans which are important for structural integrity in tissues like cartilage. It operates independently rather than as part of a complex. The proteolytic function of ADAMTS15 is essential in processes like embryonic development cell migration and angiogenesis emphasizing its influence in maintaining normal physiological functions.
Pathways
ADAMTS15 is implicated in the proteoglycan turnover pathway and the Wnt signaling pathway. Its activities within these pathways contribute to cellular matrix degradation and regulation of cell growth and differentiation. ADAMTS15 associates with other proteins such as aggrecan and collagen that participate in matrix degradation. It shows functional similarities and interactions with other ADAMTS family members like ADAMTS4 and ADAMTS5 which further support its role in matrix dynamics.
Researchers link ADAMTS15 with osteoarthritis and some forms of cancer. In osteoarthritis abnormal activity of ADAMTS15 leads to excessive degradation of cartilage matrix contributing to joint degeneration. Proteins like aggrecan targeted by ADAMTS15 play a role in this disorder. In cancer ADAMTS15 involvement appears in the regulation of tumor microenvironment dynamics and metastasis. Its interaction with structural components of the matrix makes it a potential therapeutic target in both contexts.
