Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Serum, Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.65 ng/mL,
Range:0.65 - 150 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human CRISP2 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CRISP2 in serum (human CRISP2 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants. This assay employs an antibody specific for human CRISP2 coated on a 96-well plate.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Activin RIA, ASAHL, B4GalT1, BAI1, Brorin, C1qTNF4, CA14, CA4, CA6, CA8, Cadherin-6, Caspr2, CD27 Ligand, CD300a, CD300e, CD300f, CD4, CD5, CD69, CK18, CK19, CPB1, DDR1, FUT8, MIA, NTAL, NTB-A, OMgp, PEAR1, Podoplanin, PTH1R, Reg4, ROR1, Semaphorin 4G, Serpin A5, Serpin B6, Siglec-1, Sirtuin 2, Sirtuin 5.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CRISP2 also known as Cysteine-Rich Secretory Protein 2 has a mass of approximately 25 kDa and plays an important role in sperm-egg fusion. This protein is primarily expressed in the testis and epididymis. It belongs to the CRISP family which is characterized by containing cysteine-rich sequences. Researchers have identified its expression not only in mature sperm cells but also in the lining of the ductus deferens.
Biological function summary
CRISP2 contributes to reproductive processes by being involved in sperm maturation and fertilization. It belongs to a group of proteins forming the CRISP complex which is critical in ensuring proper sperm function. The protein interacts with ion channels which suggests that it regulates ion transport processes vital for sperm activity. Researchers have shown that CRISP2 is essential for sperm-egg recognition and binding indicating its importance in fertility mechanisms.
Pathways
CRISP2 plays a significant role in the reproductive and fertilization pathways. It interacts with several ion channel proteins such as CatSper which is important for calcium ion influx in sperm cells. This interaction allows CRISP2 to influence motility and acrosome reaction important parts of the fertilization pathway. The downstream processes supported by CRISP2 engagement in pathways ensure successful fertilization by facilitating the necessary physical and chemical changes in sperm.
CRISP2 associates particularly with male infertility and certain reproductive dysfunctions. Abnormal expression of CRISP2 can lead to deficits in sperm functionality which may contribute to unexplained infertility in males. Studies have hinted at the interaction between CRISP2 and proteins like paralogous CRISP1 which may compensate or synergize in cases of CRISP2 deficiency indicating potential redundancy or interplay that influences infertility outcomes.
