Description
Product details:
These products are extremely sensitive and require specific conditions to maintain cell health and functionality. Please refer to the recommended protocols for the correct methodology in plating and maintaining these cells.
For even greater physiological relevance, co-culture protocols are available for use with Cortical astrocytes (ab315369). Co-culture with Cortical astrocytes can be performed with Cortical GABAergic Neurons, Cortical Glutamatergic Neurons (ab315366) or a combination of both in varying ratios.
Human Cortical GABAergic Neurons are derived from normal control iPSC line WC-30. BrainXell Cortical GABAergic Neurons have high neuronal purity (>80%) and comprise predominantly inhibitory neurons. Labelling with the pan-neuronal marker MAP2 and the GABA-specific marker highlights the neuronal purity of these cells. Cortical GABAergic Neurons are adherent and exhibit substantial neurite outgrowth within the first week in culture, showing the characteristic cell shape and extension of processes classic cortical GABAergic neurons. These neurons also demonstrate pronounced electrophysiological activity after two weeks in culture, as demonstrated by multi-electrode array (MEA) recordings.
Vials of Cortical GABAergic Neurons are delivered with three supplements which are designed to facilitate the maturation and health of the cultures. BrainFast GABA is a seeding supplement which promotes cell survival at thaw and completes the differentiation process from late-stage neural progenitor to neuron. BrainFast SK enhances neuronal purity, and BrainFast D4 accelerates maturation.
In partnership with BrainXell
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
