Description
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant,
Reacts with:Aequorea victoria,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.813 pg/mL,
Range:9.375 - 600 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
GFP SimpleStep ELISA
kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GFP protein in Cell culture supernatant. Quantitate GFP with 1.813 pg/ml sensitivity.
SimpleStep ELISA
technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details. Our SimpleStep ELISA
technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA
microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
