Description
Key facts
Detection method:Colorimetric,
Sample types:Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 366.056 pg/mL,
Range:937.5 - 60000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human Apolipoprotein E4 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Apolipoprotein E4 protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human Apolipoprotein E4 with 366.056 pg/ml sensitivity.
SimpleStep ELISA
technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details. Our SimpleStep ELISA
technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Apolipoprotein E4 often called ApoE4 is a member of the apolipoprotein family and plays an important role in lipid metabolism. As a 34-kDa protein it associates with lipoprotein particles and mediates receptor binding and clearance of these complexes. ApoE4 expresses mainly in the liver and the brain where it facilitates cholesterol and lipid transport and homeostasis. Other important alternative names for this protein include apo E4 and E4 peptide which identify specific functions related to its diverse roles in the body.
Biological function summary
ApoE4 is an important player in lipid transport and uptake facilitating the redistribution of lipids among cells. It forms part of a critical complex involved in binding to low-density lipoprotein receptors (LDLR) and the related protein family enabling efficient lipid delivery to peripheral tissues. The interaction of ApoE4 with these receptors directly influences cellular lipid balance impacting important cellular functions. Additionally its polymorphic nature allows the isoforms E2 E3 and E4 to differ in their impacts on lipid transport and disease risk.
Pathways
ApoE4 engages in the lipid and cholesterol transport pathways prominently impacting the reverse cholesterol transport process and the regulation of plasma lipoprotein metabolism. It interacts with proteins such as LDLR and LRP1 influencing the removal of triglyceride-rich lipoproteins and modulating lipid levels in the circulation. ApoE4's involvement in these pathways highlights its role as a central regulator of lipid homeostasis.
ApoE4 is closely associated with Alzheimer’s disease and cardiovascular disease highlighting its significance beyond lipid transport. ApoE4's isoform uniquely elevates the risk for Alzheimer's disease by potentially altering amyloid-beta metabolism and interfering with tau-associated pathologies making it a target of interest in neurodegenerative research. Additionally individuals bearing the ApoE4 allele exhibit an increased susceptibility to cardiovascular disorders due to its impact on cholesterol transport and clearance often linking it with elevated blood cholesterol levels. Through these diseases ApoE4 connects with other proteins like amyloid precursor protein (APP) and tau in Alzheimer's disease illustrating the broader implications of its biological functions.
