Description
Key facts
Assay time:2h 30m,
Assay Platform:Reagents
Product details:
RNA Fragmentation Reagent Kit (ab323459) is a reagent set used to fragment isolated RNA and purified mRNA for downstream processes including RNA-seq and next generation sequencing. The kit provides a simple, reliable and fast method for RNA library preparation.
Isolated purified RNA is incubated with divalent cations at 94°C to fragment RNA into shorter oligonucleotide strands. The fragment size of RNA can be controlled by extending or shortening the incubation time and stopping the reaction with Fragmentation Stop Solution.
Prepare 1 µg of isolated RNA samples in PCR Tubes with 10X Fragmentation Buffer and incubate in thermal cycler at 94°C for 1-5 minutes to fragment RNA. Add 10X Fragmentation Stop Solution to samples to stop the fragmentation reaction. Clean up fragmented RNA by performing an Ethanol precipitation. Fragmented samples may be qualified using gel electrophoresis to assess the yield and size distribution of fragmented RNA samples.
Performing a DNase-treatment on isolated RNA at sample prep is recommended for optimal results. Removal of buffer salts from fragmented RNA can be achieved by standard cold ethanol precipitation.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
