Key facts
Host species:Alpaca,
Target species:Mouse,
Target isotype:IgG1,
Target specificity:Heavy chain,
Pre-adsorbed:No,
Conjugation:Alexa Fluor® 647,
Excitation/Emission:Ex: 650nm, Em: 665nm,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Clonality:Monoclonal,
Clone number:10A4,
Isotype:IgG,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.
Product details:
FluoTags®
FluoTags are fully defined & ready-to-go fluorescent camelid single-domain antibody (sdAb) conjugates. All FluoTags® carry a defined number of fluorophores at known positions. Accordingly, they are available as FluoTag®-X2 or FluoTag®X4.
Together with their monovalent binding mode, the defined fluorophore conjugation of FluoTags® results in a more quantitative and reliable readout in all fluorescence-based analyses. Due to the small size the fluorophore displacement from the bound target protein is very low and therefore FluoTags® are ideal for standard super resolution technologies.
FluoTag®-X2 (One target - One sdAb – Two fluorophores (1:1:2)
FluoTag®-X2 reagents are composed of a single sdAb carrying two fluorophores per protein molecule. The maximal displacement of fluorophores from the antigen-binding surface is ~3 nm. FluoTag®-X2 reagents show good tissue penetration features.
Features:
High affinity monovalent binding + two fluorophores per sdAb for brighter signals
Reagent is made from small-sized proteins. This ensures optimal access to the respective target protein, also in crowded environment or 3D samples.
Smart Secondaries®
Smart Secondaries® are a unique set of high-affinity single-domain antibodies recognizing conventional immunoglobulins with exquisite species and isotype specificity. They provide unique benefits for all antibody-based assays in research and diagnostics by combining reproducible specificity and production with small and high-affinity monovalent binding. The technology offers surprising new possibilities like one-step indirect immunofluorescence or easy multiplexing.
In comparison to conventional secondary reagents, Smart Secondary® nanobodies feature significant advantages:
Small probes for enhanced staining speed and improved tissue penetration and higher resolution.
Monoclonal tools: Clearly defined species specificity – once and forever!
Isotype specificity for independent detection of mouse IgG1 and IgG2a/b isotypes.
Recombinant production for reliable quality, scalability and reproducibility without using animals.
Monovalent binding mode – Pre-incubation without clustering primary antibodies and therefore one-step indirect immunofluorescence possible!
Extraordinarily strong binding allows one-step multi-colour staining and imaging even with primary antibodies from identical species and isotype (Multiplexing)
Equipped with propriety FX technology for improved post-fixation and enhanced performance in demanding application (e.g., expansion microscopy, DNA-PAINT, dSTORM, etc.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or
[email protected].
Properties and Storage Information:
Form-Liquid, Purification technique-Size-exclusion chromatography, Storage buffer-Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1 month, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle
