Description
Key facts
Host species:Alpaca,
Target species:Rabbit,
Target isotype:IgG,
Target specificity:Fc region,
Pre-adsorbed:No,
Conjugation:AZdye 568,
Excitation/Emission:Ex: 578nm, Em: 602nm,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Clonality:Multiclonal,
Clone number:10E10 / 8C10,
Isotype:IgG,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.
Product details:
FluoTags®
FluoTags are fully defined & ready-to-go fluorescent camelid single-domain antibody (sdAb) conjugates. All FluoTags® carry a defined number of fluorophores at known positions. Accordingly, they are available as FluoTag®-X2 or FluoTag®X4.
Together with their monovalent binding mode, the defined fluorophore conjugation of FluoTags® results in a more quantitative and reliable readout in all fluorescence-based analyses. Due to the small size the fluorophore displacement from the bound target protein is very low and therefore FluoTags® are ideal for standard super resolution technologies.
FluoTag®-X4 (One target - Two sdAb – Four fluorophores (1:2:4)
Each FluoTag®-X4 reagent is a blend of two different sdAbs (FluoTag®-X2 and FluoTag®-X2-B) molecules that independently bind the target protein. As each sdAb carries two fluorophores per protein molecule, the blend can direct up to 4 fluorophores to each target protein. The reagent is therefore up to 2-fold brighter than the corresponding FluoTag®-X2 products.
The maximal displacement of fluorophores from each antigen-binding surface is ~3 nm. FluoTag®-X4 reagents have the same size as the corresponding FluoTag®-X2 products and therefore show similarly good tissue penetration features.
Features:
A blend of two high affinity monovalent sdAbs directs four fluorophores to each target protein – ideal for bright signals
Small size for optimal access to the respective target protein, also in crowded environment or 3D samples
Smart Secondaries®
Smart Secondaries® are a unique set of high-affinity single-domain antibodies recognizing conventional immunoglobulins with exquisite species and isotype specificity. They provide unique benefits for all antibody-based assays in research and diagnostics by combining reproducible specificity and production with small and high-affinity monovalent binding. The technology offers surprising new possibilities like one-step indirect immunofluorescence or easy multiplexing.
In comparison to conventional secondary reagents, Smart Secondary® nanobodies feature significant advantages:
Small probes for enhanced staining speed and improved tissue penetration and higher resolution.
Monoclonal tools: Clearly defined species specificity – once and forever!
Isotype specificity for independent detection of mouse IgG1 and IgG2a/b isotypes.
Recombinant production for reliable quality, scalability and reproducibility without using animals.
Monovalent binding mode – Pre-incubation without clustering primary antibodies and therefore one-step indirect immunofluorescence possible!
Extraordinarily strong binding allows one-step multi-colour staining and imaging even with primary antibodies from identical species and isotype (Multiplexing)
Equipped with propriety FX technology for improved post-fixation and enhanced performance in demanding application (e.g., expansion microscopy, DNA-PAINT, dSTORM, etc.
Properties and Storage Information:
Form-Liquid, Purification technique-Size-exclusion chromatography, Storage buffer-Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1 month, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle
