Description
Key facts
Host species:Alpaca,
Target species:Mouse,
Target isotype:IgG2a, IgG2b,
Target specificity:Heavy chain hinge,
Pre-adsorbed:No,
Conjugation:AZdye 568,
Excitation/Emission:Ex: 578nm, Em: 602nm,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Clonality:Monoclonal,
Clone number:14A4,
Isotype:IgG,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.
Product details:
FluoTags®
FluoTags are fully defined & ready-to-go fluorescent camelid single-domain antibody (sdAb) conjugates. All FluoTags® carry a defined number of fluorophores at known positions. Accordingly, they are available as FluoTag®-X2 or FluoTag®X4.
Together with their monovalent binding mode, the defined fluorophore conjugation of FluoTags® results in a more quantitative and reliable readout in all fluorescence-based analyses. Due to the small size the fluorophore displacement from the bound target protein is very low and therefore FluoTags® are ideal for standard super resolution technologies.
FluoTag®-X2 (One target - One sdAb – Two fluorophores (1:1:2)
FluoTag®-X2 reagents are composed of a single sdAb carrying two fluorophores per protein molecule. The maximal displacement of fluorophores from the antigen-binding surface is ~3 nm. FluoTag®-X2 reagents show good tissue penetration features.
Features:
High affinity monovalent binding + two fluorophores per sdAb for brighter signals
Reagent is made from small-sized proteins. This ensures optimal access to the respective target protein, also in crowded environment or 3D samples.
Smart Secondaries®
Smart Secondaries® are a unique set of high-affinity single-domain antibodies recognizing conventional immunoglobulins with exquisite species and isotype specificity. They provide unique benefits for all antibody-based assays in research and diagnostics by combining reproducible specificity and production with small and high-affinity monovalent binding. The technology offers surprising new possibilities like one-step indirect immunofluorescence or easy multiplexing.
In comparison to conventional secondary reagents, Smart Secondary® nanobodies feature significant advantages:
Small probes for enhanced staining speed and improved tissue penetration and higher resolution.
Monoclonal tools: Clearly defined species specificity – once and forever!
Isotype specificity for independent detection of mouse IgG1 and IgG2a/b isotypes.
Recombinant production for reliable quality, scalability and reproducibility without using animals.
Monovalent binding mode – Pre-incubation without clustering primary antibodies and therefore one-step indirect immunofluorescence possible!
Extraordinarily strong binding allows one-step multi-colour staining and imaging even with primary antibodies from identical species and isotype (Multiplexing)
Equipped with propriety FX technology for improved post-fixation and enhanced performance in demanding application (e.g., expansion microscopy, DNA-PAINT, dSTORM, etc.
Properties and Storage Information:
Form-Liquid, Purification technique-Size-exclusion chromatography, Storage buffer-Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1 month, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle
