Description
Key facts
Detection method:Colorimetric,
Sample types:Tissue Lysate, Cell Lysate,
Assay type:Enzyme activity,
Assay time:2h,
Assay Platform:Microplate reader
Product details:
Caspase-3 Assay Kit (Colorimetric) ab39401 allows the rapid, sensitive and accurate measurement of Caspase-3 activity in cell lysates.
How the assay works
The Caspase-3 assay protocol is based on the formation of the chromophore p-nitroaniline (p-NA) by cleavage from the labeled substrate DEVD-pNA. The p-NA can be quantified using a spectrophotometer or a microtiter plate reader reading absorbance at 400 or 405 nm.
Comparison of the absorbance of p-NA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-3 activity.
Caspase-3 Assay protocol summary
- Add samples to wells
- Add reaction buffer and DEVD-p-NA substrate and incubate for 60-120 min at 37°C
- Analyze with microplate reader
Note: Due to the nature of the substrate, this assay also detects caspase-7 activity.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
