Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 15 pg/mL,
Range:56.25 - 1800 pg/mL,
Assay time:1h 45m,
Assay Platform:Microplate
Product details:
Abcam's CD130 (GP130) Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD130 in Human serum, plasma, buffered solutions and supernatants.
A monoclonal antibody specific for CD130 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD130 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD130 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD130 present in the samples.
This kit will recognize both endogenous and recombinant Human CD130.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD130 also known as gp130 or the IL-6 signal transducer (IL6ST) is a protein weighing around 130 kDa. This receptor is encoded by the IL6ST gene and widely expressed on the surface of cells across multiple tissues. CD130 functions mechanically as a signal transducing receptor facilitating the assembly of receptor complexes necessary for the initiation of signal transduction. It plays a major role in cytokine receptor signaling particularly interacting with interleukin-6 (IL-6) family cytokines.
Biological function summary
CD130 functions as part of the high-affinity receptor complex for certain cytokines. It partners with other receptors such as CD126 to form a complex that initiates signaling pathways after ligand binding contributing to a range of immune responses. Its role is thorough across many cellular processes including differentiation proliferation and apoptosis. In addition to immune signaling CD130 is involved in several growth factor signaling pathways.
Pathways
CD130 serves a significant role in signal transduction particularly in the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway. It works in concert with related proteins like JAK1 and STAT3 to transmit signals from extracellular cytokines to the nucleus influencing gene expression. Additionally CD130 can also be found active in the MAPK/ERK pathway which is important for cellular response to growth signals.
CD130 plays a connection especially to inflammatory conditions and certain cancers. Aberrant signaling involving CD130 has been linked to disorders like rheumatoid arthritis where it contributes to the inflammation process. Additionally it relates to multiple myeloma a cancer where abnormal CD130 interactions with proteins like IL-6 may enhance cell survival and proliferation presenting potential targets for therapeutic intervention.
