Description
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag C-Terminus,
Applications:ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P29990,
Animal free:No,
Carrier free:No,
Species:Dengue virus 2 Thailand/16681/84,
Storage buffer:pH: 7.2 - 7.6Constituents: 48% Urea, 1.58% Tris HCl, 1.16% Sodium chloride
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Dengue Virus NS1 glycoprotein also known as NS1 alternative DN1 and others like BC5 and CC6 is a non-structural protein that plays an important role in dengue virus infection. It has a molecular mass of about 46-50 kDa and expresses in both intracellular and extracellular environments. Within infected cells NS1 produces in the endoplasmic reticulum (ER). Outside the cells it exists as a soluble hexamer and gets secreted into the bloodstream.
Biological function summary
Within infected cells NS1 aids dengue virus replication and contributes to viral RNA synthesis. Though not part of a ribonucleoprotein complex NS1 interacts with the viral RNA and RNA polymerase facilitating efficient genome replication. It also interferes with the host’s immune response helping the virus escape immune detection. The extracellular form of NS1 triggers immune responses and can lead to vascular leakage by disrupting endothelial cell function.
Pathways
NS1 significantly impacts the flavivirus replication cycle and the host's immune pathways. It interacts with proteins such as FB10 in the replication pathway affecting replication efficiency. In the immune pathway NS1 modulation impairs the complement system through interactions with proteins like OB4 reducing the host's ability to clear the virus.
The NS1 protein closely links to dengue fever and dengue hemorrhagic fever. It associates with the severity of these diseases due to its role in immune evasion and vascular permeability. NS1 levels in circulation correlate with disease severity and interactions with DN2 contribute to immune modulation exacerbating symptoms and leading to complications in infected individuals.
