Description
Key facts
Detection method:Fluorescent,
Sample types:Tissue Extracts, Cell Lysate,
Assay type:Enzyme activity,
Assay time:2h,
Assay Platform:Microplate reader
Product details:
Cathepsin K Activity Assay Kit (Fluorometric) (ab65303) is a fluorescence-based assay that utilizes the preferred cathepsin K substrate sequence LR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin K will cleave the synthetic substrate LR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader.
This product is manufactured by BioVision, an Abcam company and was previously called K141 Cathepsin K Activity Fluorometric Assay Kit. K141-100 is the same size as the 100 test size of ab65303.
Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cathepsin K also known as CTSK is a protease enzyme that belongs to the papain family of cysteine proteases. With a molecular weight of approximately 37 kDa Cathepsin K is mainly expressed in osteoclasts which are cells involved in bone resorption. This enzyme functions by cleaving collagen a vital component of bone extracellular matrix which facilitates bone remodeling and turnover. Known for its collagenolytic activity Cathepsin K is important in processes where breakdown of collagen fibers is required.
Biological function summary
Cathepsin K plays a significant role in bone metabolism. It operates within the osteoclasts where it degrades type I collagen and other matrix proteins essential for normal bone maintenance and repair. Cathepsin K does not function as part of a complex but acts independently in its enzymatic role. It is highly specific to substrates found in the bone matrix differentiating it from other cathepsins like cathepsin L or B that may have broader expression and role.
Pathways
Cathepsin K is an important player in the bone remodeling pathway. This pathway involves a series of coordinated actions between osteoclasts and osteoblasts to maintain bone health. Cathepsin K's activity is tightly regulated within this pathway to ensure proper bone density and structure. It is associated with other proteins such as osteopontin and matrix metalloproteinases which work together to modulate bone turnover. Within the RANK/RANKL pathway Cathepsin K acts as a downstream effector following osteoclast activation by RANKL.
Cathepsin K is most notably linked to osteoporosis and pycnodysostosis. Osteoporosis arises due to excessive bone resorption where elevated Cathepsin K activity leads to weakened bone structure increasing fracture risk. Pycnodysostosis is a rare genetic disorder caused by mutations in the gene coding for Cathepsin K resulting in impaired bone resorption and abnormally dense but brittle bones. In these conditions proteins such as RANKL and osteoprotegerin also play critical roles by influencing osteoclast differentiation and activity thereby modulating Cathepsin K's effects.
