Description
Key facts
Detection method:Colorimetric,
Sample types:Urine, Plasma, Tissue Extracts, Serum, Other biological fluids, Cell Lysate,
Assay type:Enzyme activity,
Sensitivity:< 4 mU/well,
Assay time:1h 30m,
Assay Platform:Microplate reader
Product details:
GST Assay Kit (Colorimetric) (ab65326) is used to detect GST activity in crude cell lysates and purified protein fractions, and also to quantitate GST-tagged fusion proteins.
The GST assay protocol is based on the GST-catalyzed reaction between GSH and the GST substrate CDNB.
CDNB has the broadest range of isozyme detectability (e.g., alpha-, mu-, pi-, and other GST isoforms). The GST-catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected by spectrophotometer at 340 nm.
One unit of GST activity is defined as the amount of enzyme producing 1 umol of GS-DNB conjugate/min under the conditions of the assay. Detection limit of assay: active GST < 1 mU.
GST assay protocol summary:
- add samples and positive control to wells
- add glutathione to wells
- add reaction mix
- analyze with a microplate reader for at least 10 min
Other Notes
This product was previously called K263 Biovision GST Colorimetric Activity Assay Kit. Biovision was acquired by Abcam in 2021.
Glutathione S-transferase (GST, EC 2.5.1.13) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GST or Glutathione S-transferase is an enzyme family known for detoxifying endogenous and exogenous compounds through conjugation with glutathione. GSTs are about 23–28 kDa in size and are expressed in various tissues such as the liver kidneys and lungs. The GST proteins play vital roles in cellular processes and are utilized extensively in molecular biology often tagged to proteins to enhance solubility known as GST-tagged proteins.
Biological function summary
GST enzymes participate in critical cellular detoxification processes enhancing the solubility of toxins. As part of these processes GST enzymes do not function as part of a larger complex but are individual actors within the cell. They facilitate the conjugation reactions fundamental to cellular metabolism and detoxification making them essential for maintaining cellular health.
Pathways
GST enzymes contribute significantly to the metabolic breakdown pathways like the xenobiotic metabolism pathway. This pathway involves proteins such as cytochrome P450 enzymes which often function upstream to modify compounds for further GST action. GSTs are important for efficiently detoxifying potentially harmful substances preventing cellular damage.
GSTs have considerable relevance to cancer where their overexpression can confer drug resistance in tumor cells complicating treatment approaches. Additionally these enzymes connect with proteins such as MDR1 influencing multidrug resistance in cancer therapy. GSTs also relate to asthma where altered GST activity can affect the body's response to oxidative stress highlighting the enzyme's role in inflammatory processes.
