Key facts
Detection method:Colorimetric/Fluorometric,
Sample types:Heparin Plasma, Tissue Extracts, Serum, Other biological fluids, Cell Lysate, Urine (UTI),
Assay type:Quantitative,
Sensitivity:> 2 µM,
Assay time:1h 20m,
Assay Platform:Microplate reader
Product details:
Triglyceride Assay Kit (ab65336) is a sensitive, easy assay to measure triglyceride concentration in mammalian samples.
Other names used to refer to this type of assay include TAG assay, TG assay, and triacylglycerol assay.
How the Triglyceride assay works
In the triglyceride assay protocol, triglycerides are converted to free fatty acids and glycerol by a lipase enzyme. Glycerol is then phosphorylated by glycerol kinase, and subsequently oxidized by glycerol phosphate oxidase to produce hydrogen peroxide. The hydrogen peroxide reacts with a probe via a peroxidase to generate color (spectrophotometry at λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm).
Triglyceride assay protocol summary
- Add samples and standards to wells.
- Add assay buffer and lipase, and incubate for 20 min.
- Add triglyceride reaction mix and incubate for 60 min.
- Analyze with microplate reader.
Getting the best performance from ab65336
Please note: the general range is 0-10 nmol (colorimetric) and 0-1 nmol (fluorometric).
If your sample contains reducing substances, they are likely to interfere with the assay. In this case, we recommend using Triglyceride Assay Kit (Fluorometric, Reducing samples)
ab178780
Technical Note: Triglyceride is unlikely to be detectable in healthy urine but may be elevated in the presence of infection or disease of the urinary tract.
How other researchers are using Triglyceride Assay Kit ab65336
The Triglyceride assay kit has been used in publications in a variety of sample types, including:
- Human: serum
, plasma
, mammary epithelial and mammary cancer cell line lysate
, Huh7.5 hepatocyte-derived cell line lysate
, primary liver cell line lysates
, sebocyte cell culture lysates
- Mouse: hepatocyte cell lysates
, liver extract
, serum
, plasma
, kidney extracts
, liver tissue and serum
, cardiac tissue extracts
- Rat: liver tissue extract
, plasma
- Drosophila
References: 1-Huang Y et al. 2019, PMID: 30778327, 2-Wilson et al 2018; PMID: 29534545, 3-Yen MC et al. 2019, PMID: 30911270, 4-Kim D et al. 2018, PMID: 30127285, 5-Boteon Y et al. 2018, PMID: 30044872, 6- Jin S and Lee MY 2018, PMID: 30400322, 7-Zhang W et al. 2019, PMID: 30664220, 8-Brial F et al. 2019, PMID: 30842494, 9-Liu J et al. 2018, PMID: 30344653 and Patton A et al. 2018, PMID: 29666152, 10-Fernandes et al. 2018, PMID: 29320745 and Körholz JC et al 2018, PMID: 30362941 and Liang et al. 2018, PMID: 29659562, 11-Ding W et al. 2018, PMID: 29563333, 12-Cui XB et al. 2018, PMID: 29449334, 13-Rohm M et al. 2018<. PMID: 29610263, 14-Yu S et al. 2018, PMID: 29867579, 15-García-Ruiz et al. 2018, PMID: 29367725, 16-Wen CA and Ballard JWO 2019, PMID: 30805163
Related and recommended products
Triglyceride assay kit ab65336 is often used with Free Fatty Acid assay kit
ab65341
, and Cholesterol assay kit
ab65390
, to study lipid metabolism and its role in various metabolic diseases such as diabetes, obesity, liver diseases, and cardiovascular conditions.
Other notes
This product is manufactured by BioVision, an Abcam company and was previously called K622 Triglyceride Quantification Colorimetric/Fluorometric Kit. K622-100 is the same size as the 100 test size of ab65336.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Triglycerides also known as triacylglycerols are a type of lipid molecule found mainly in adipose tissue and the bloodstream. They consist of three fatty acids bonded to a glycerol backbone leading to a total molecular mass that varies based on the fatty acid chain lengths. Triglycerides originate in different tissues like the liver and intestines. The enzyme lipase acts on triglycerides breaking them down into free fatty acids and glycerol which is essential for metabolic processes. Scientists often measure triglyceride levels in blood to assess lipid metabolism and related health conditions.
Biological function summary
Triglycerides serve as a major form of energy storage in the body. They are important substrates for energy production especially during fasting or prolonged physical activity when the body's glycogen stores are depleted. Triglycerides do not function independently but as part of lipoprotein complexes. These complexes transport lipids throughout the body in the bloodstream. Understanding the normal triglyceride range helps in assessing if levels are within a healthy limit indicating effective lipid metabolism. Average triglyceride levels may vary but maintaining a good triglyceride level is important for overall health.
Pathways
Triglycerides are involved in lipid metabolism and energy homeostasis. One important pathway includes the lipolysis pathway where triglycerides undergo breakdown via lipase into fatty acids and glycerol. This reaction is important for providing energy particularly involving proteins such as hormone-sensitive lipase (HSL) and adipose triglyceride lipase (ATGL). In another pathway triglycerides play a role in the synthesis and storage of fats influenced by proteins like peroxisome proliferator-activated receptors (PPARs) which regulate gene expression involved in fatty acid storage.
High triglyceride levels relate to cardiovascular disease and pancreatitis. Triglyceride-rich lipoproteins link to atherosclerosis making the regulation of triglycerides important for heart health. Chronic high levels may lead to acute pancreatitis by causing free fatty acid accumulation damaging pancreatic cells. Proteins such as apolipoprotein C-III affect triglyceride metabolism and may influence the risk of developing cardiovascular diseases. Effective management of triglyceride levels can help reduce these health risks.
