Description
Key facts
Detection method:Colorimetric/Fluorometric,
Sample types:Other biological fluids, Serum, Urine,
Assay type:Quantitative,
Assay time:1h,
Assay Platform:Microplate reader
Product details:
Creatinine Assay Kit ab65340 provides an accurate, convenient measure of creatinine concentration in biological fluids such as serum, urine or CSF.
How the assay works
In the creatinine assay protocol, creatinine is converted to creatine by creatininase, creatine is converted to sarcosine by creatinase. Sarcosine is then oxidized by sarcosine oxidase producing hydrogen peroxide. Hydrogen peroxide is then processed by a peroxidase causing a reaction with a probe to generate red color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm).
Creatinine assay protocol summary
- Add samples and standards to wells.
- Add reaction mix and incubate for 60 min at 37°C.
- Analyze with microplate reader.
Getting the best performance from ab65340
Unlike picric acid assays, this kit is suitable for serum creatinine determinations, as well as for urine and other biological samples.
For deproteinization of samples: Better results are typically seen with this assay when using a 10kda filter for sample deproteinization than when using the PCA method.
Related and recommended products
Creatinine assay kit ab65340 is commonly used by researchers to measure kidney function.
Kidney function marker assays include:
- Creatinine Assay Kit ab65340 - suitable for use as a serum creatinine assay and a urine creatinine assay.
- Mouse Albumin ELISA Kit
ab108792
and Human Albumin ELISA Kit
ab227933
– suitable for use with serum/plasma and urine
- Albumin (BCG) Assay Kit (Colorimetric)
ab235628
– suitable for use with serum
- Urea Assay Kit
ab83362
– suitable for use with serum/plasma and urine, and used as a blood urea nitrogen assay.
The albumin-creatinine ratio is often measured by using in combination Creatine Assay Kit ab65340, and either Mouse Albumin ELISA Kit
ab108792
or Human Albumin ELISA Kit
ab227933
The urea-creatinine ratio is often measured by using in combination Urea Assay Kit
ab83362
and Creatinine Assay Kit ab65340.
Albumin Creatinine Ratio Assay Kit
ab241018
is suitable for urine and serum, and combines a fluorescent albumin assay with a colorimetric creatinine assay in a convenient pack.
Creatinine assay methods
There are two established methods of creatinine assays:
- Jaffe assay
The Jaffe assay method is based on the reaction of creatinine with picric acid at an alkaline pH. The creatinine‐picrate complex has an absorbance of 500 nm.
- Enzymatic creatinine assay
The standard enzymatic creatinine assay uses the method described above for ab65340.
The Jaffe method is substantially simpler and cheaper than enzymatic creatinine assays, however it suffers substantially from interference by metabolites found in urine and serum.
Other notes
This product is manufactured by BioVision, an Abcam company and was previously called K625 Creatinine Colorimetric/Fluorometric Assay Kit. K625-100 is the same size as the 100 test size of ab65340.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Creatinine also known as 2-amino-1-methyl-5H-imidazol-4-one is a small molecule with a molecular mass of approximately 113.12 g/mol. Its mechanical functions stem from its role as a breakdown product of creatine phosphate primarily in muscle. Creatinine serves as a marker for kidney function due to its constant production and relatively stable concentration in the blood. It gets filtered out of the blood by the kidneys and is present in urine. Laboratories focus on measuring creatinine levels using tools like the creatinine assay kit and creatinine test kit to evaluate renal function.
Biological function summary
In the human body creatinine plays an integral role in reflecting muscle metabolism rates and biochemical balance. Creatinine levels aid in assessing glomerular filtration rate (GFR) an important measure of kidney function. It is not part of a protein complex as it results from muscle activity. Its levels in blood and urine help calculate the protein to creatinine ratio providing insights into renal health. The constant exchange and excretion of creatinine make it a reliable biomarker in metabolism studies.
Pathways
Muscle metabolism and kidney filtration both involve creatinine's function. Creatinine formation and elimination connect to pathways that maintain homeostasis and waste removal. Key proteins in these pathways include creatine kinase which catalyzes the conversion of creatine to creatine phosphate. The creatinine protein ratio becomes relevant in evaluating proteinuria a condition where excess protein is present in urine indicating possible kidney dysfunction.
Kidney-related conditions and muscle disorders often involve creatinine. Common health concerns like chronic kidney disease (CKD) associate with elevated creatinine levels indicating reduced kidney function. Additionally conditions such as muscular dystrophy can alter muscle creatinine levels. These diseases relate to proteins such as nephrin involved in the kidney filtration barrier and dystrophin essential for muscular integrity. For clinical assessments tools like the creatinine assay and understanding the protein creatinine ratio are key for early detection and management.
