Description
Key facts
Detection method:Colorimetric/Fluorometric,
Sample types:Urine, Plasma, Cell culture extracts, Tissue Extracts, Serum, Other biological fluids,
Assay type:Quantitative,
Sensitivity:> 1 µM,
Range:1 - 200 µM,
Assay time:40m,
Assay Platform:Microplate reader
Product details:
Pyruvate Assay Kit (Colorimetric/Fluorometric) ab65342 provides a simple, direct and automation-ready procedure for measuring pyruvate concentration in various biological samples such as blood, cells, culture and fermentation media, etc.
In the pyruvate assay protocol, pyruvate is oxidized by pyruvate oxidase via enzyme reactions to generate color (λ= 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to pyruvate content, the pyruvate concentration can be accurately measured.
The kit detects 1-200 μM pyruvate.
Pyruvate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at room temp
- analyze with microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K609 Pyruvate Colorimetric/Fluorometric Assay Kit. K609-100 is the same size as the 100 test size of ab65342.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pyruvate often called pyruvic acid or 'pyr' is an important intermediate metabolite in cellular respiration. It has a molecular mass of 88.06 g/mol and plays an integral role in the metabolic process. Pyruvate is present in virtually all living cells including plants and animals. The compound forms during glycolysis through the breakdown of glucose and can undergo several conversions serving as a precursor for various biochemical pathways. Potassium pyruvate is the salt form commonly used to study pyruvate's functions in the lab.
Biological function summary
This compound serves essential roles in energy production and biosynthesis. As part of the pyruvate dehydrogenase complex pyruvate connects glycolysis to the Krebs cycle by enabling the conversion to acetyl-CoA. This conversion is necessary for the aerobic respiration process. Furthermore pyruvate contributes to gluconeogenesis where it influences the creation of glucose from non-carbohydrate sources important during periods of fasting or intense exercise.
Pathways
Pyruvate interacts significantly in metabolic pathways like glycolysis and the citric acid cycle emphasizing cellular energy metabolism. In glycolysis pyruvate acts as the endpoint molecule while in the citric acid cycle it enters as acetyl-CoA maintaining the cycle in motion. The regulation of these pathways involves various enzymes and proteins like pyruvate kinase and lactate dehydrogenase associate with pyruvate’s metabolic roles dictating transitions between aerobic and anaerobic states.
Alterations in pyruvate metabolism link closely to cancer and diabetes. Cancer cells often exhibit increased glycolytic rates known as the Warburg effect where pyruvate's conversion influences tumor growth. Pyruvate kinase M2 a protein variant is notably expressed in cancer cells and affects pyruvate's fate. In diabetes impaired pyruvate metabolism impacts insulin regulation contributing to the disease's metabolic complications. High levels of pyruvate can lead to lactic acidosis emphasizing the balance required in normal metabolic functioning.
