Description
Key facts
Detection method:Colorimetric,
Sample types:Urine, Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate,
Assay type:Enzyme activity,
Sensitivity:> 1 µM,
Range:0.001 - 1 mM,
Assay time:2h,
Assay Platform:Microplate reader
Product details:
Phosphate Assay Kit (Colorimetric) (ab65622) provides an easy, quick and simple method for measuring phosphate levels.
The phosphate assay protocol uses a proprietary formulation of malachite green and ammonium molybdate which forms a chromogenic complex with phosphate ion giving an intense absorption band around OD = 650nm.
Phosphate assay protocol summary:
- add reaction mix to sample and standard wells
- incubate for 30 min
- analyze with a microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K410 Phosphate Colorimetric Assay Kit. K410-500 is the same size as the 500 test size of ab65622.
This assay can be used with biological fluids but also inorganic samples such as algal blooms and water from run-off areas of high fertilizer use.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient, Storage information-Ambient
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Phosphate a simple inorganic chemical functions mechanically as a component of various essential biological molecules such as nucleic acids ATP and phospholipids. Phosphate ions serve as an important player in cellular energy transfer through involvement in phosphorylation reactions. It's present abundantly in living organisms especially in the form of calcium phosphate in bones and teeth. The molecular weight of a phosphate ion is approximately 94.97 g/mol. Expressed ubiquitously across different tissues and cells phosphate is also available in multiple assay formats like the phosphate assay kit and phosphorus assay kits for scientific research.
Biological function summary
Phosphate plays a role in numerous cellular processes beyond its participation in energy metabolism. It contributes critically to cellular signaling pathways as a part of second messengers like cyclic AMP and is a core part of protein kinase and phosphatase complexes which regulate many cellular functions. Moreover phosphate acts in buffering systems maintaining cellular pH balance. In the cellular context the probe and colorimetric malachite green assay often quantify phosphate levels aiding in understanding phosphate homeostasis in biochemical studies.
Pathways
The chemical phosphate is integral to significant pathways such as glycolysis and the citric acid cycle where it assists in energy production and storage. It associates with proteins like hexokinase in the glycolytic pathway and is critical for the formation of high-energy the phosphate bonds in ATP. As phosphate cycles through these pathways it continuously modulates various metabolic activities and biochemical reactions within the cell highlighting its universal importance.
Phosphate imbalances relate to conditions such as chronic kidney disease and hypophosphatemia. In chronic kidney disease altered phosphate homeostasis can lead to cardiovascular complications due to phosphate's interactions with proteins such as fibroblast growth factor 23 (FGF23) which regulates phosphate metabolism. In hypophosphatemia a deficiency in phosphate can result in skeletal disorders highlighting the need for precise phosphate detection and monitoring in clinical settings.
