Description
Key facts
Detection method:Colorimetric/Fluorometric,
Sample types:Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids,
Assay type:Quantitative,
Sensitivity:< 0.007 nmol/well,
Assay time:40m,
Assay Platform:Microplate reader
Product details:
Galactose Assay Kit (Colorimetric/Fluorometric) (ab83382) provides a simple and convenient method for direct measurement of galactose levels in various biological samples such as serum, plasma and other body fluids, as well as food and growth media. In this assay, oxidation of galactose generates a product that reacts with the galactose probe to produce color (OD570nm) or fluorescence (Ex/Em 535/587 nm). Liquid samples can be tested directly without purification. The assay is fast, convenient and sensitive. It can also be used as a high throughput assay.
High concentrations of lactose could result in elevated readings for free galactose. Galactose oxidase is not completely selective, and it can react with the galactose moiety of lactose.
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This product is manufactured by BioVision, an Abcam company and was previously called K621 Galactose Colorimetric/Fluorometric Assay Kit. K621-100 is the same size as the 100 test size of ab83382.
Galactose (C
MW: 180.16 g/mol) is one of the major naturally occurring sugars, a monosaccharide that differs from glucose only by the configuration of the hydroxyl group at the carbon-4 position. Often present as an anomeric mixture of α-D-galactose and β-D-galactose, this monosaccharide exists abundantly in milk, dairy products and many other food types such as fruits and vegetables.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Galactose a simple sugar with a molecular weight of 180.16 g/mol is a monosaccharide similar to glucose. It serves as an energy source and forms part of glycoproteins and glycolipids. In the human body the liver ovaries and seminal vesicles express galactose. Cells in these tissues utilize galactose for various cellular activities contributing to functions like cell-cell recognition and signaling. Common assays measure galactose concentrations to understand metabolic conditions and tools like galactose assay kits offer specific insights.
Biological function summary
Galactose plays a critical role in the Leloir pathway converting galactose into glucose-1-phosphate. This conversion involves several enzymes including galactokinase and uridylyltransferase. Not existing within any larger complex galactose activity supports the proper metabolism of carbohydrates and the synthesis of complex biomolecules. Its function ensures proper cellular metabolism by providing precursors for energy production and biosynthesis.
Pathways
Galactose contributes significantly to the glycolysis and gluconeogenesis processes. UDP-galactose an activated form of galactose interacts in these metabolic pathways highlighting its role in carbohydrate metabolism. The protein galactokinase is essential in phosphorylating galactose for its subsequent incorporation into these pathways. Another protein galactose-1-phosphate uridylyltransferase further catalyzes the transformation of galactose derivatives showing the interconnectedness of these proteins within metabolic networks.
Defective galactose metabolism leads to conditions like galactosemia a genetic disorder affecting an enzyme in the Leloir pathway. Classical galactosemia involves mutations in the galactose-1-phosphate uridylyltransferase gene causing an accumulation of galactose-1-phosphate in tissues and organs leading to toxicity. A related condition congenital cataracts can arise from galactose pathway disruptions where crystallins proteins in the lens of the eye are affected by elevated galactitol resulting from excess galactose. Understanding galactose's biological role helps monitor and manage these disorders effectively.
