Description
Key facts
Detection method:Colorimetric,
Sample types:Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids,
Assay type:Quantitative,
Sensitivity:> 0.01 mM,
Range:0.01 - 0.2 mM,
Assay time:40m,
Assay Platform:Microplate reader
Product details:
beta Hydroxybutyrate (beta HB) Assay Kit (Colorimetric) (ab83390) utilizes beta HB Dehydrogenase to generate a product which reacts with a colorimetric probe with an absorbance band at 450 nm.
The kit is an easy and accurate assay to measure beta HB levels in biological samples. The assay is linear for 1-20 nmol beta HB in up to 100 μL samples or 0.01-0.2 mM of beta HB samples.
beta Hydroxybutyrate assay protocol summary
- add reaction mix to sample and standard wells
- incubate for 30 min
- analyze with microplate reader
Related and recommended products
The term 'Ketone bodies' refers to three chemicals, acetoacetate, beta hydroxybutyrate and acetone. Ketone bodies are produced by the liver and used peripherally as an energy source when glucose is not readily available. The two main ketone bodies are acetoacetate (AcAc) and 3-β-hydroxybutyrate (3HB), while acetone is the third, and least abundant, ketone body.
This assay kit can be used to measure the beta hydroxybutyrate element of ketone bodies.
To measure both the beta hydroxybutyrate and acetoacetate elements of ketone bodies in a single assay kit, use Ketone Body Assay Kit
ab318308
Acetoacetate Assay Kit
ab180875
uses the non-enzymatic nitroferricyanide method to measure the acetoacetate element of ketone bodies.
Other Notes
This product is manufactured by BioVision, an Abcam company and was previously called K632 β-Hydroxybutyrate (β-HB) Colorimetric Assay Kit. K632-100 is the same size as the 100 test size of ab83390.
During diabetic ketoacidosis the liver produces ketone bodies (acetoacetate, acetone, and primarily beta-hydroxybutyrate) from the acetyl CoA produced from fatty acid oxidation. The very high rate of ketone body production outstrips the body's ability to utilize them as an energy source and the blood concentration builds up. As rather strong acids, they result in a significant drop in blood pH.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Beta-Hydroxybutyrate also called β-hydroxybutyrate or B-hydroxybutyrate serves as an important ketone body in the metabolism of energy. It has a molecular mass of approximately 104.1 g/mol. Cells in the liver particularly during periods of low glucose availability produce beta-hydroxybutyrate from acetyl-CoA derived from fatty acid oxidation. The liver then releases it into the bloodstream where peripheral tissues such as the brain heart and skeletal muscle can utilize it as an energy source. Researchers commonly use beta-hydroxybutyrate assays to quantify its levels in serum or plasma providing valuable information about metabolic status and energy balance.
Biological function summary
Beta-hydroxybutyrate functions as both a fuel molecule and a signaling molecule. It is part of the ketone body complex which also includes acetoacetate and acetone. It assists in providing energy to tissues during fasting or ketogenic diets by entering cells and converting back to acetyl-CoA which enters the citric acid cycle. Additionally beta-hydroxybutyrate influences gene expression inflammation and oxidative stress by interacting with receptors and modulating pathways at the cellular level.
Pathways
Beta-hydroxybutyrate is involved in the ketogenesis and ketolysis pathways. During ketogenesis liver cells convert fatty acids to ketone bodies including beta-hydroxybutyrate which is then transported to extrahepatic tissues. In these tissues ketolysis occurs refashioning the ketone body back to acetyl-CoA. The enzyme 3-hydroxybutyrate dehydrogenase plays an important role in this process by interconverting beta-hydroxybutyrate and acetoacetate. These pathways are closely linked to gluconeogenesis and glycolysis underlining the significance of beta-hydroxybutyrate in energy homeostasis.
Beta-hydroxybutyrate levels are highly relevant in conditions like diabetes and epilepsy. In diabetes particularly in type 1 diabetic ketoacidosis elevated beta-hydroxybutyrate levels result from uncontrolled ketogenesis due to insufficient insulin highlighting metabolic dysregulation. Meanwhile in epilepsy ketogenic diets aim to stabilize neuronal activity by increasing beta-hydroxybutyrate levels which has been observed to reduce seizure frequency. Proteins such as insulin in diabetes and glutamate transporters in epilepsy play roles in these contexts showing the diverse impact of beta-hydroxybutyrate on health and disease.
