Description
Key facts
Detection method:Colorimetric/Fluorometric,
Sample types:Tissue Lysate, Food samples, Cell Lysate,
Assay type:Quantitative,
Sensitivity:> 4 µg/mL,
Range:4 - 2000 µg/mL,
Assay time:40m,
Assay Platform:Microplate reader
Product details:
Abcam's Starch Assay Kit provides an easy, accurate assay to measure starch levels in a variety of samples. In the assay, starch is hydrolyzed to glucose which is oxidized to generate color (570 nm) and fluorescence (Ex/Em = 535/587 nm). The assay can detect starch at 0.0004 to 2 mg/ml.
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This product is manufactured by BioVision, an Abcam company and was previously called K647 Starch Colorimetric/Fluorometric Assay Kit. K647-100 is the same size as the 100 test size of ab83393.
Starch is a complex carbohydrate consisting of a large number of glucose units. All plants contain starch, present as amylose, (linear α-1,4 linked polymer) and amylopectin, (highly α-1,6 branched α-1,4 polymer). Starch generally contains 0-25% amylose and 75-100% amylopectin.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Starch is a polysaccharide consisting of numerous glucose units joined together by glycosidic bonds. It is commonly found in plants and serves as a major form of energy storage. Starch comprises two components: amylose which is mostly linear and amylopectin which is highly branched. Its molecular mass varies depending on the ratios of amylose to amylopectin. Starch accumulates in various plant tissues particularly in seeds tubers and roots where it functions as a reserve of carbohydrates to be used when photosynthesis is not possible.
Biological function summary
Starch plays a significant role as an energy source in the dietary intake of humans and animals. It is part of the carbohydrate storage complex in plants. Animals and humans break down starch using specific enzymes such as amylase and maltase converting it into glucose for energy growth and cellular function. This stored energy becomes essential for processes such as germination in plants and demands of high metabolic activities in animals. Starch's role in nutrition is critical among both domestic and wild species.
Pathways
Starch is involved in key metabolic routes such as the glycolysis and gluconeogenesis. In animals and humans enzymes like alpha-amylase initiate its breakdown paving the way for glucose to enter glycolytic pathways for ATP production whereas plant metabolism involves enzymes such as starch synthase within chloroplasts. Glycolysis enables efficient energy release from the stored glucose derived from starch linking to insulin and other regulatory proteins thereby integrating with broader metabolic networks.
Starch digestion-related abnormalities such as congenital sucrase-isomaltase deficiency and type 2 diabetes have notable connections. In the case of diabetes improper starch metabolism leads to hyperglycemia due to inadequate insulin activity or production and involves proteins such as insulin and glucagon. These disorders highlight the importance of controlled starch breakdown and absorption processes in maintaining normal physiological functions highlighting the potential for starch-related interventions in disease management.
