Description
Key facts
Detection method:Colorimetric/Fluorometric,
Sample types:Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids,
Assay type:Quantitative,
Sensitivity:> 24 µg/mL,
Range:24 - 76 µg/mL,
Assay time:40m,
Assay Platform:Microplate reader
Product details:
Abcam's Alanine Assay Kit provides a sensitive detection method of alanine. In the kit, alanine is converted to pyruvate which is specifically detected leading to proportional color (λ=570nm: 0-10 nmol) or fluorescence (Ex/Em 535/587nm: 0-1 nmol) development. Serum concentration: ~24-76 μg/ml (~3-9 nmol/10 μl).
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This product is manufactured by BioVision, an Abcam company and was previously called K652 Alanine Colorimetric/Fluorometric Assay Kit. K652-100 is the same size as the 100 test size of ab83394.
Alanine is the 2nd most abundant of the 20 proteinogenic amino acids. Nonessential, being available from dietary sources, it plays a key role in the glucose-alanine cycle between tissues and liver. In muscle and other tissues that degrade amino acids, amino groups are pooled as glutamate by transamination. Glutamate then transfers the amino group to pyruvate via alanine aminotransferase, forming alanine and α-ketoglutarate. The alanine is passed into the blood and transported to the liver. A reverse of the alanine aminotransferase reaction takes place in liver. Pyruvate can be used in gluconeogenesis, to form glucose which may return to other tissues through the circulatory system. There appears to be a correlation between alanine levels and and higher blood pressure, energy intake, cholesterol levels, and body mass index.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alanine also known as L-alanine is a non-essential amino acid with a molecular mass of 89.09 g/mol. Alanine can be found in high concentrations in muscle tissue and is one of the building blocks of proteins in many tissues and organs. L-alanine’s functional group includes an amino group and a carboxyl group which gives it properties important for metabolic processes. It operates as a part of the alanine cycle and is often involved in the transamination process which involves the transfer of an amino group from one molecule to another.
Biological function summary
Alanine contributes to several important metabolic functions in the body by functioning as an energy source for muscles. Alanine does not form part of any major complexes but often acts as a medium for the transportation of nitrogen and carbon across tissues. Within the liver it participates in gluconeogenesis where it can be converted to glucose providing energy during periods of fasting or physical exertion.
Pathways
The involvement of alanine in the glucose-alanine cycle and the Krebs cycle highlights its significance in metabolic activity. This cycle facilitates the exchange of metabolites between the muscle and liver providing a means to balance nitrogen waste and energy production. Alanine is closely related to other amino acids such as glutamine and glutamate as these are key players in nitrogen metabolism and energy transactions within cells.
Studies connect alanine to conditions like type 2 diabetes and certain amino acid metabolic disorders. The imbalance in alanine levels affects glucose metabolism influencing insulin sensitivity and glycemic control. Alanine relates to proteins like insulin in diabetes showing the potential connection between amino acid metabolism and endocrine function. Understanding alanine's role provides insights into therapeutic targets for related metabolic diseases.
