Description
Key facts
Species or organism:Human,
Form:LiquidSee storage information
Product details:
ab94309 is a 293T cell transfected lysate in which Human Exonuclease 1 has been transiently over-expressed using a pCMV-Exonuclease 1 plasmid. The lysate is provided in 1X Sample Buffer.
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Exonuclease 1 (Exo1) also known simply as Exonuclease I is a protein playing a significant mechanical role in DNA processes. The protein functions by degrading nucleic acid molecules from their ends specifically acting on the 5' ends to progressively remove nucleotides. Exo1 exhibits specificity for double-stranded DNA and participates mainly in DNA repair and recombination. The molecular weight of Exo1 is approximately 94 kDa. Expression of Exo1 occurs in various tissues with prevalent expression in tissues undergoing active cell division.
Biological function summary
Exo1 is key in maintaining genomic stability. This protein engages in homologous recombination mismatch repair and the resection process during DNA double-strand break repair. Exo1 works collaboratively as part of larger complexes particularly interacting with proteins like MRE11-RAD50-NBS1 (MRN) complex in DNA repair mechanisms. The interaction within these complexes enhances its efficacy in processing DNA ends and facilitates accurate repair.
Pathways
Exo1 is integral to the DNA repair and homologous recombination pathways. In the DNA repair pathway Exo1 functions in conjunction with proteins such as MutL homologs and DNA polymerases ensuring proper mismatch repair by creating single-strand gaps. Through homologous recombination Exo1 links with proteins such as BRCA1 and RAD51 facilitating the repair of double-strand breaks by processing the DNA ends to allow strand invasion and exchange steps of recombination.
Exo1 has connections with cancer and hereditary nonpolyposis colorectal cancer (HNPCC). In cancer impaired or mutated Exo1 can lead to defective DNA repair mechanisms resulting in genomic instability and tumorigenesis. The protein shows linkage with MLH1 in HNPCC as both participate in mismatch repair. Faulty interactions or mutations in either Exo1 or associated proteins like MLH1 or MSH2 can contribute to the pathogenesis of these conditions highlighting the gene-disease relationship within the context of DNA repair processes.
