Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 20 pg/mL,
Range:27.4 - 20000 pg/mL,
Assay Platform:Microplate
Product details:
Abcam's ARP4 (Angiopoietin-like 4) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human ARP4 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human ARP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and ARP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human ARP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ARP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimisation may be required with urine samples.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ARP4 protein also known as Actin-Related Protein 4 plays a role in chromatin remodeling which is important for DNA-related processes like transcription replication and repair. ARP4 has a molecular mass of approximately 50 kDa and is ubiquitously expressed in eukaryotic cells. It remains localized mainly within the nucleus where it contributes to changes in the configuration of chromatin structure permitting the necessary access of various transcription machinery to DNA.
Biological function summary
ARP4 functions as an integral component of the INO80 chromatin remodeling complex. This complex is essential for modifying chromatin structure which impacts gene expression regulation and DNA repair. Through its association with the INO80 complex ARP4 contributes to the mobilization and spacing of nucleosomes important for maintaining genomic stability. It also further enhances DNA repair processes by allowing more accessible interactions with DNA sites requiring repair.
Pathways
ARP4 is involved in the regulation of gene expression and DNA damage response. The protein plays a part in the chromatin remodeling pathway and the DNA repair pathway. Through its work with the INO80 complex ARP4 associates with other remodelers like RUVBL1 and RUVBL2 which are also components of the same complex. These interactions facilitate effective transcriptional regulation and the DNA damage response showcasing the critical nature of ARP4 in maintaining normal cellular functions.
ARP4 has a place in connecting with conditions like cancer and neurodegenerative diseases. Aberrant expression or mutations in ARP4 and its associated complexes can disrupt chromatin structure leading to misregulation of gene expression which can precipitate tumorigenesis or neuronal damage. Through its impact on chromatin remodeling and gene regulation ARP4 works alongside other proteins like BRG1 a part of the SWI/SNF chromatin remodeling complex influencing pathways that may result in these diseases.
